Influence of Preservation Solutions and Bile on a Human Bile Duct Epithelium Cell Line: An In-Vitro Study

Philipp Stiegler, Ursula Mayrhauser, Sonja Koestenbauer, Bettina Leber, Katja Konrad, Florian Iberer, Karlheinz Tscheliessnigg, Vanessa Stadlbauer

Division of Internal Medicine, Department of Gastroenterology and Hepatology, Medical University Graz.
Division of Surgery, Department for Transplantation Surgery, Medical University Graz.

DOI: 10.4236/ojots.2012.24010                

Abstract

Introduction: Bile duct complications are common after liver transplantation. The impact of preservation solution is unclear. Aim: We investigated the impact of different preservation solutions with and without diluted bile on a human biliary tract carcinoma cell line. Methods: The human biliary tree carcinoma cell line SK-ChA-1 was cultured with either medium or University of Wisconsin solution (UW), histidine-tryptophane-ketoglutarate (HTK) solution, Celsior or physiological saline for 6h, 10h or 12h at 6℃- 8℃ and metabolic activity was measured by a MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)-test immediately, after 12h or 24h. Cells were also incubated in combination with diluted porcine bile. Results: Immediately after cold storage of 6h HTK and UW decreased metabolic activity whereas Celsior increased metabolic activity after 10h or 12h of cold ischemia. After 12h or 24h no major differences were found any more. Incubation with bile in combination with HTK, Celsior or NaCl decreased metabolic activity, whereas UW abolished this effect. Conclusion: On a cellular level differences between preservation solutions were found, especially in combination with bile. Further studies are warranted to determine whether this results in clinically significant differences in biliary complications.

Keywords

Organ Preservation; Liver Transplantation; Bile Duct Epithelium; In Vitro

Conflicts of Interest

The authors declare no conflicts of interest.

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