Activities of Some Nigerian Medicinal Plants against Aedes aegypti


Extracts and constituents of medicinal plants have proven to be biodegradable, had low mammalian toxicity and induction of resistance, and comparable activities to the standard drugs. Therefore, methanolic extracts of some plants that are termite resistant or used ethnomedically as antimalarial and febrifuge were evaluated for activities against 4th-instar larvae of Aedes aegypti. A 61 % of these plants with these properties demonstrated larvicidal activities and may confirm the usefulness of these properties in choosing plant larvicides. This is the first report of larvicidal activities of stem barks and leaves of Blighia sapida and Baphia nitida, stem barks of Markhamia tomentosa and Newboldia laevis, and whole plants of Euphorbia macrophylla. Extracts of B. sapida stem bark, Costus specious root and Xylopia aethiopica seed, with LC50 1.71, 1.47 and 1.49 mg/ml at 48 h, respectively, were the most active and had significant activities that were comparable to Endosulphan. Hence, they may be used as plant larvicides in the control of dengue and yellow fever.

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A. Adebajo, F. Famuyiwa, J. John, E. Idem and A. Adeoye, "Activities of Some Nigerian Medicinal Plants against Aedes aegypti," Chinese Medicine, Vol. 3 No. 3, 2012, pp. 151-156. doi: 10.4236/cm.2012.33024.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] G. N. Grantz, “What Must We Do to Effectively Control Aedes aegypti?” Journal of Tropical Medicine, Vol. 35, No. 4, 1993, pp. 243-251.
[2] V. Prajapati, A. K. Tripathi, K. K. Aggarwal and S. P. S. Khanuja, “Insecticidal, Repellent and Oviposition-Deterrent Activity of Selected Essential Oils against Anopheles stephensi, Aedes aegypti and Culex quinquefas-ciatus,” Bioresource Technology, Vol. 96, No. 16, 2005, pp. 1749-1757. doi:10.1016/j.biortech.2005.01.007
[3] L. Donno, “Malaria: Prevalence and Distribution,” In: Malaria, Montedison Group, Farmitalia Carlo Erba, Milan, 1984, pp. 7-13.
[4] J. A. Rozendaal, “Mosquitoes and other Biting Diptera,” In: Vector Control, World Health Organization, Geneva, Switzerland, 1997, pp. 5-177.
[5] J. E. Casida and G. B. Quistad, “Insecticide Targets: Learning to Keep up with Resistance and Changing Concepts of Safety,” Agricultural Chemistry and Biotechnology, Vol. 43, No. 4, 2000, pp. 185-191.
[6] J. B. Lima, M. P. Da-Cunha, R. C. Da Silva, A. K. Galardo, S. S. Soares, I. A. Braga et al., “Resistance of Aedes aegypti to Organophosphates in Several Municipalities in the State of Rio de Janeiro and Espírito Santo, Brazil,” American Journal of Tropical Medicine and Hygiene, Vol. 68, No. 3, 2003, pp. 329-333.
[7] F. Chandre, F. Darriet, M. Darder, A. Cuany, J. M. C. Doannio, N. Pasteur, et al., “Pyrethroid Resistance in Culex quinquefasciatus from West Africa,” Medical and Veterinary Entomology, Vol. 12, No. 4, 1998, pp. 359-366. doi:10.1046/j.1365-2915.1998.00120.x
[8] R. P. Penilla, A. D. Rodriguez, J. Hemingway, J. L. Torres, J. I. Arredondo-Jimenez and M. H. Rodriguez, “Resistance Management Strategies in Malaria Vector Mosquito Control. Baseline Data for a Large-Scale Field Trial against Anopheles albimanus in Mexico,” Medical and Veterinary Entomology, Vol. 12, No. 3, 1998, pp. 217- 233. doi:10.1046/j.1365-2915.1998.00123.x
[9] T. Su and M. S. Mulla, “Ovicidal Activity of Neem Products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae),” Journal of American Mosquito Control Association, Vol. 14, No. 2, 1998, pp. 204-209.
[10] W. Choochote, D. Kanjanapothi, A. Panthong, T. Taesotikul, A. Jitpakdi, U. Chaithong, et al., “Larvicidal, Adulticidal and Repellent Effects of Kaempferia galangal,” Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 30, No. 3, 1999, pp. 470-476.
[11] A. M. Arriaga, J. Q. Lima, J. N. e Vasconcelos, M. C. de Oliveira, T. L. Lemos, A. M. Fonseca, et al., “Antioxidant and Larvicidal Activities of Tephrosia egregia Sandw against Aedes aegypti,” Natural Products Communications, Vol. 4, No. 4, 2009, pp. 529-530.
[12] S.-S. Cheng, H.-T. Chang, C.-Y. Lin, P.-S. Chen, C.-G. Huang, W.-J. Chen, et al., “Insecticidal Activities of Leaf and Twig Essential Oils from Clausena excavata against Aedes aegypti and Aedes albopictus Larvae,” Pest Management Science, Vol. 65, No. 3, 2008, pp. 339-343. doi:10.1002/ps.1693
[13] L. C. Ming, “Ageratum conyzoides: A Tropical Source of Medicinal and Agricultural Products,” In: J. Janick, Ed., Perspectives on New Crops and New Uses, ASHS Press, Alexandria, 1999, pp. 469-473.
[14] S. A. Mitchell and M. H. Ahmad, “A Review of Medicinal Plant Research at the University of West Indies, Jamaica,” West Indian Medical Journal, Vol. 55, No. 4, 2001, pp. 243-269.
[15] C. Orwa, A. Mutua, R. Kindt, R. Jamnadass and S. Anthony, “Agroforestree Database: A Tree Reference and Selection Guide Version 4.0,” World Agroforestry Centre, Kenya, 2009.
[16] J. O. Igoli, O. G. Ogaji, T. A. Toranyiin and N. P. Igoli, “Traditional Medicine Practice amongst the Igede People of Nigeria. Part II,” African Journal of Traditional, Complementary and Alternative Medicine, Vol. 2, No. 2, 2005, pp. 134-152.
[17] F. Tantangmo, B. N. Lenta, F. F. Boyom, S. Ngouela, M. Kaiser, E. Tsamo, et al., “Antiprotozoal Activities of Some Constituents of Markhamia tomentosa (Bignoniaceae),” Annals of Tropical Medicine and Parasitology, Vol. 104, No. 5, 2010, pp. 391-398. doi:10.1179/136485910X12743554760180
[18] J. Kayode, “Conservation of Indigenous Medicinal Botanicals in Ekiti State, Nigeria,” Journal of Zhejiang University Science B, Vol. 7, No. 9, 2006, pp. 713-718. doi:10.1631/jzus.2006.B0713
[19] R. P. Umbare, G. S. Mate1, D. V. Jawalkar, S. M. Patil and S. S. Dongare, “Quality Evaluation of Phyllanthus amarus (Schumach) Leaves Extract for its Hypolipidemic Activity,” Biology and Medicine, Vol. 1, No. 4, 2009, pp. 28-33.
[20] H. M. Burkill, “The Useful Plants of West Tropical Africa,” Kew Publishing, Royal Botanical Gardens, Kew, 1985, pp. 11-20.
[21] A. Sofowora, “Medicinal Plants and Traditional Medicine in Africa,” Spectrum Books Ltd., Nigeria, 1993, pp. 142-157.
[22] E. A. Ross, “Medicinal Plants of the World: Chemical Constituents, Traditional and Modern Medicinal Uses,” Humana Press, Totowa, Vol. 2, 2001, p. 487.
[23] V. M. Couto, F. C. Vilela, D. F. Dias, M. H. Dos Santos, R. Soncini, C. G. Nascimento, et al., “Antinociceptive Effect of Extract of Emilia sonchifolia in Mice,” Journal of Ethnopharmacology, Vol. 134, No. 2, 2011, pp. 348-353. doi:10.1016/j.jep.2010.12.028
[24] B. A. Ayinde and F. Odigie, “Larvicidal Properties of the Volatile Oil of Ageratum conyzoides L. (Compositae) against Culex specie Mosquito Larvae,” Nigerian Journal of Applied Science, Vol. 19, 2001, pp. 23-25.
[25] E. T. Oparaocha, I. Iwu and J. E. Ahanaku, “Preliminary Study on Mosquito Repellent and Mosquitocidal Activities of Ocimum gratissimum (L.) Grown in Eastern Nigeria,” Journal of Vector Borne Diseases, Vol. 47, No. 1, 2010, pp. 45-50.
[26] WHO, “Guidelines for Laboratory and Field Testing of Mosquito Larvicides,” World Heath Organisation Communicable Disease Control, Prevention and Eradication - WHO Pesticide Evaluation Scheme, 2005, pp. 10-11.
[27] G. F. Ibikunle, A. C. Adebajo, F. G. Famuyiwa, A. J. Aladesanmi and C. O. Adewunmi, “In Vitro Evaluation of Anti-Trichomonal Activities of Eugenia uniflora Leaf,” African Journal of Traditional, Complementary and Alternative Medicine, Vol. 8, No. 2, 2011, pp. 170-176.
[28] A. A. Rahuman, G. Gopalakrishnan, P. Venkatesan and K. Geetha, “Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae),” Parasitology Research, Vol. 102, No. 5, 2008, pp. 867-873. doi:10.1007/s00436-007-0839-6
[29] M. E Macêdo, R. A. G. B. Consoli, T. S. M. Grandi, A. M. G. dos Anjos, A. B. de Oliveira, N. M. Mendes et al., “Screening of Asteraceae (Compositae) Plant Extracts for Larvicidal Activity against Aedes fluviatilis (Diptera: Culicidae),” Mems do Instituto Ostwaldo Cruz, Rio de Janeiro, Vol. 92, No. 4, 1997, pp. 565-570.
[30] A. A. S. Amusan and O. A. Oke, “The Toxicity of Hexanolic Extract of Xylopia aethiopica to Laboratory Reared Larvae of Cx. p. quiquefasciatus,” Proceedings of the International Conference on Science & National Development, 25-28 October, 2004, College of Natural Sciences Proceedings, Federal University of Agriculture, Abeokuta, Nigeria, pp. 109-112.
[31] C. Kamaraj, A. A. Rahuman, A. Mahapatra, A. Bagavan and G. Elango, “Insecticidal and Larvicidal Activities of Medicinal Plant Extracts against Mosquitoes,” Parasitology Research, Vol. 107, No. 6, 2010, pp. 1337-1349. doi:10.1007/s00436-010-2006-8
[32] S. Promsiri, A. Naksathit, M. Kruatrachue and U. Thavara, “Evaluations of Larvicidal Activity of Medicinal Plant Extracts to Aedes aegypti (Diptera: Culicidae) and other Effects on a non Target Fish,” Insect Science, Vol. 13, 2006, 179-188. doi:10.1111/j.1744-7917.2006.00080.x
[33] J. Reisch, A. C. Adebajo, A. J. Aladesanmi, S. K. Adesina, D. Bergenthal and U. Meve, “Chemotypes of Murraya koenigii growing in Sri Lanka,” Planta Medica, Vol. 60, No. 3, 1994, pp. 295-296. doi:10.1055/s-2006-959486
[34] N. Chungsamarnyart, S. Jiwajinda and W. Jansawan, “Larvicidal Effect of Plant Crude-Extracts on the Tropical Cattle Tick (Boophiltus microplus),” Kasetsart Journal: Natural Science, Vol. 25, 1991, pp. 80-89.
[35] M. R. Dowlathabad, G. SapthaJyothi, R. M. Reddy, M. V. V. Prasad and K. Subramanyam, “Larvicidal Activity of Essential Oils from Indian Medicinal Plants against Aedes aegypti L.,” Journal of Pharmacy Research, Vol. 2, No. 4, 2009, pp. 762-764.
[36] B. Pitasawat, W. Choocote, D. Kanjanapothi, A. Panthong, A. Jitpakdi and U. Chaithong, “Screening for Larvicidal Activity of Ten Carminative Plants,” Southeast Asian Journal of Tropical Med Public Health, Vol. 29, No. 3, 1998, pp. 660-662.
[37] S. M. Habeeb, A. H. El-Namaky and M. A. Salama, “Efficiency of Allium. cepa and Commiphora molmol as a Larvicidal Agent against Fourth Stage Larvae of C. pipiens (Diptera: Culicidae),” American-Eurasian Journal of Agriculture & Environmental Science, Vol. 5, No. 2, 2009, pp.196-203.
[38] S. S. Ranaweera, “Mosquito-Larvicidal Activity of some Sri Lankan Plants,” Journal of the National Science Council of Sri Lanka, Vol. 24, No. 2, 1996, pp. 63-70.

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