Sensitivity assay of polymerase chain reaction for detection of Canine Parvo Virus infection in dogs

DOI: 10.4236/ojcd.2012.23009   PDF   HTML   XML   3,724 Downloads   7,148 Views   Citations

Abstract

A polymerase chain reaction was performed using re-ported primers for detection of Canine Parvo virus (CPV) in the stool sample obtained from repository. The PCR primers were specific to VP1/VP2 gene of CPV. Sensi-tivity assay of PCR detection was performed by making dilutions of CPV positive DNA extracted from fecal sample, carrying out PCR for each dilution and visualiz-ing amplicons in ethidium bromide stained agarose gel under UV radiation. Study was valuable in determining the efficiency of PCR. The sensitivity of PCR in present study was determined to be equivalent to detection of .00 2pg/μl of CPV DNA. The study was conducted to analyze the variation, sensitivity and repeatability.

Share and Cite:

Sharma, P. , Rastogi, A. , Kukreti, K. and Narwal, P. (2012) Sensitivity assay of polymerase chain reaction for detection of Canine Parvo Virus infection in dogs. Open Journal of Clinical Diagnostics, 2, 45-47. doi: 10.4236/ojcd.2012.23009.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Sagazio, P., Tempesta, M., Buonavoglia, D., Cirone, F. and Bounavoglia, C. (1998) Antigenic characterization of canine parvovirus strains isolated in Italy. Journal of Virological Methods, 73, 197-200. doi:10.1016/S0166-0934(98)00055-X
[2] Ramadass, P. and Khader, T.G.A. (1982) Diagnosis of canine parvovirus infection by agar gel precipitation test and fluorescent antibody techniques. Cherion, 11, 323-328.
[3] Kumar and Dharmadheeran (2008) Detection of CPV from fecal samples by PCR. Journal of Remount and Veterinary Corps, 47, 197-201.
[4] Narayanan, S., Amit, K., Chug, P.K., et al. (2001) Strain characterization of Indian isolates of Canine Parvo Virus by PCR RFLP. Journal of Remount and Veterinary Corps, 40, 67-74.

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.