Development of a Behavioral and Imaging Model of Feeding in Planarians

DOI: 10.4236/pp.2012.33049   PDF   HTML   XML   4,469 Downloads   7,215 Views  

Abstract

Excess weight is a major risk factor for type-2 diabetes, cardiovascular disease, and other comorbidities. Animal models of feeding provide insight into the problem and provide a means for the discovery and evaluation of pharmacotherapeutic treatment. Mammalian models are the most commonly used, but recently non-mammalian models have been utilized (e.g., C. elegans). Planarians provide an intermediate model. They are the earliest extant animal with a primitive brain-like structure and are a convenient model of mammalian behavioral endpoints and drug-induced effects. The purpose of the present study was to determine if a quantitative measure of presumptive feeding behavior could be visualized using an imaging technique. Colored food pellets were prepared and in some experiments, plasmid-delivered green fluorescent protein was added. Both visible and fluorescence microscopy displayed clear indication of internalization of the red dye and localization to the digestive system. This new methodology establishes a convenient way to study normal physiological feeding behavior as well as modifications induced by drugs or other exogenous substances.

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R. B. Raffa, C. S. Tallarida, S. R. Patel, S. M. Rawls and E. Krynetskiy, "Development of a Behavioral and Imaging Model of Feeding in Planarians," Pharmacology & Pharmacy, Vol. 3 No. 3, 2012, pp. 364-367. doi: 10.4236/pp.2012.33049.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] NIH, “Publication No. 98-4083: NHLBI Obesity Education Initiative. Clinical Guidelines on the Identification, Evaluation, and Treatment of Overweight and Obesity in Adults,” 1998. http://www.nhlbi.nih.gov/guidelines/obesity/ob_gdlns.pdf
[2] C. L. Ogden, M. D. Carroll, B. K. Kit and K. M. Flegal, “Prevalence of Obesity in the United States,” NCHS Data Brief, No 82, National Center for Health Statistics, Hyattsville, 2009-2010.
[3] P. T. Katzmarzyk and S. A. Lear, “Physical Activity for Obese Individuals: a Systematic Review of Effects on Chronic Disease Risk Factors,” Obesity Reviews, Vol. 13, No. 2, 2012, pp. 95-105. doi:10.1111/j.1467-789X.2011.00933.x
[4] R. P. Troiano, E. A. Frongillo Jr., J. Sobal and D. A. Levitsky, “The Relationship Between Body Weight and Mortality: a Quantitative Analysis of Combined Information From Existing Studies,” International Journal of Obesity & Related Metabolic Disorders, Vol. 20, No. 1, 1996, pp. 63-75.
[5] A. M. Wolf and G. A. Colditz, “The Cost of Obesity: US Perspective,” Pharmacoeconomics, Vol. 5, Suppl. 1, 1994, pp. 34-37. doi:10.2165/00019053-199400051-00007
[6] J. M. McGinnis and W. H. Foege, “Actual Causes of Death in the United States,” Journal of the American Medical Association, Vol. 270, No. 18, 1993, pp. 2207- 2212. doi:10.1001/jama.1993.03510180077038
[7] S. Xie, M. A. Furjanic, J. J. Ferrara, N. R. McAndrew, E. L. Ardino, A. Ngondara, Y. Bernstein, K. J. Thomas, E. Kim, J. M. Walker, S. Nagar, S. J. Ward and R. B. Raffa, “The Endocannabinoid System and Rimonabant: A New Drug With a Novel Mechanism of Action Involving Cannabi-noid CB1 Receptor Antagonism—Or Inverse Agonism—As Potential Obesity Treatment and Other Therapeutic Use,” Journal of Clinical Pharmacy and Therapeutics, Vol. 32, No. 3, 2007, pp. 209-231. doi:10.1111/j.1365-2710.2007.00817.x
[8] S. J. Ward and R. B. Raffa, “Rimonabant Redux and Strategies to Improve the Future Outlook of CB1 Receptor Neutral-Antagonist/Inverse-Agonist Therapies,” Obesity, Vol. 19, No. 7, 2011, pp. 1325-1334. doi:10.1038/oby.2011.69
[9] R. B. Raffa and S. J. Ward, “CB1-Independent Mechanisms of Δ9-THCV, AM251 and SR141716 (Rimonabant),” Journal of Clinical Pharmacy and Therapeutics, Vol. 37, No. 3, 2012, pp. 260-265.
[10] K. Traynor, “Panel Advises Against Ri-monabant Approval,” American Journal of Health System Pharmacists, Vol. 64, No. 14, 2007, pp. 1460-1461. doi:10.2146/news070065
[11] J. Zheng and F. L. Greenway, “Caenorhabditis Elegans as a Model for Obesity Research,” International Journal of Obesity, Vol. 36, No. 2, 2012, pp. 186-194. doi:10.1038/ijo.2011.93
[12] R. B. Raffa and S. M. Rawls, “Planaria: A Model for Drug Action and Abuse,” Landes Bioscience, Austin, 2008.
[13] R. B. Raffa, K. E. Finno, C. S. Tallarida and S. M. Rawls, “Topiramate-Antagonism of L-Glutamate-Induced Paroxysms in Planarians,” European Journal of Pharmacology, Vol. 649, No. 1-3, 2010, pp. 150-153. doi:10.1016/j.ejphar.2010.09.021
[14] M. S. Phadke, N. F. Krynetskaia, A. K. Mishra and E. Krynetskiy, “Glyce-raldehyde 3-Phosphate Dehydrogenase Depletion Induces Cell Cycle Arrest and Resistance to Antimetabolites in Human Carcinoma Cell Lines,” Journal of Pharmacology and Experimental Therapeutics, Vol. 331, No. 1, 2009, pp. 77-86. doi:10.1124/jpet.109.155671
[15] H. Li, P. N. Black and C. C. DiRusso, “A Live-Cell High-Throughput Screening Assay for Identification of Fatty Acid Uptake Inhibitors,” Annals of Biochemistry, Vol. 336, No. 1, 2005, pp. 11-19. doi:10.1016/j.ab.2004.09.025
[16] K. Ashrafi, F. Y. Chang, J. L. Watts, A. G. Fraser, R. S. Kamath, J. Ahringer and G. Ruvkun, “Genome-Wide RNAi Analysis of Caenorhabditis Elegans Fat Regulatory Genes,” Nature, Vol. 421, No. 6920, 2003, pp. 268-272. doi:10.1038/nature01279
[17] D. Hirsch, A. Stahl and H. F. Lodish, “A Family of Fatty Acid Transporters Conserved From Mycobacterium to Man,” Proceedings of the National Academy of Science (USA), Vol. 95, No. 15, 1998, pp. 8625-8629. doi:10.1073/pnas.95.15.8625
[18] K. Yen, T. T. Le, A. Bansal, S. D. Narasimhan, J. X. Cheng and H. A. Tissen-baum, “A Comparative Study of Fat Storage Quantitation in Nematode Caenorhabditis Elegans Using Label and Label-Free Methods,” PLoS One, Vol. 5, No. 9, 2010, p. e12810. doi:10.1371/journal.pone.0012810
[19] D. S. Dwyer, D. Donohoe, X. H. Lu and E. J. Aamodt, “Mechanistic Connections between Glucose/Lipid Disturbances and Weight Gain Induced by Antipsychotic Drugs,” International Review of Neurobiology, Vol. 65, 2005, pp. 211-247.
[20] S. M. Rawls, T. Patil, C. S. Tallarida, S. Baron, M. Kim, K. Song, S. Ward and R. B. Raffa, “Ni-cotine Behavioral Pharmacology: Clues from Planarians,” Drug and Alcohol Dependence, Vol. 118, No. 2-3, 2011, pp. 274-279. doi:10.1016/j.drugalcdep.2011.04.001
[21] C. A. Vyas, S. M. Rawls, R. B. Raffa and J. G. Shackman, “Glutamate and Aspartate Measurements in Individual Planaria by Rapid Capillary Electrophoresis,” Journal of Pharmaco-logical and Toxicological Methods, Vol. 63, No. 1, 2011, pp. 119-122. doi:10.1016/j.vascn.2010.08.002
[22] S. M. Rawls, T. Patil, E. Yuvasheva and R. B. Raffa, “First Evidence That Drugs of Abuse Produce Behavioral Sensitization and Cross Sensitization in Planarians,” Behavioral Pharmacology, Vol. 21, No. 4, 2010, pp. 301-313. doi:10.1097/FBP.0b013e32833b0098
[23] S. M. Rawls, T. Thomas, M. Adeola, T. Patil, N. Ray-mondi, A. Poles, M. Loo and R. B. Raffa, “Topiramate Antagonizes NMDA- and AMPA-Induced Seizure-Like Activity in Planarians,” Pharmacology Biochemistry and Behavior, Vol. 93, No. 4, 2009, pp. 363-367. doi:10.1016/j.pbb.2009.05.005
[24] S. M. Rawls, T. Gomez, Z. Ding and R. B. Raffa, “Differential Behavioral Effect of the TRPM8/TRPA1 Channel Agonist Icilin (AG-3-5),” European Journal of Pharmacology, Vol. 575, No. 1-3, 2007, pp. 103-104. doi:10.1016/j.ejphar.2007.07.060

  
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