Evaluation of Factors Influencing the Biomass of Soil Microorganisms and DNA Content


The aim of the study was the statistical evaluation of the impact of water potential (pF), oxygen availability (ODR) and the way of land use on microbial biomass (MB) and soil DNA content. Soil was extracted from the surface (0 - 20 cm) and subsurface (20 - 40 cm) layers of Mollic Gleysol. Soil material was collected in July 2009 from the village Kosiorow (SE part of Poland), from the two distinct neighbouring areas: agriculturally exploited (AE), and fallow land (FL), which served as the control area. Moisture content was determined for a range of pF values (0, 1.0, 1.5, 2.0), which corresponded to availability of water usable by microorganisms and plant roots. Finally, our results revealed significant (p < 0.001) positive relationship between DNA and soil MB content, and negative correlations between soil MB and both pF and ODR. Importantly, MB seemed significantly dependent on the different way of land use, and higher MB content was noted in the soil agriculturally exploited (p < 0.05) in contrast to the control area.

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W. Agnieszka, S. Zofia, B. Aleksandra and B. Artur, "Evaluation of Factors Influencing the Biomass of Soil Microorganisms and DNA Content," Open Journal of Soil Science, Vol. 2 No. 1, 2012, pp. 64-69. doi: 10.4236/ojss.2012.21010.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] K. Broos, M. L. Macdonald, D. A. Heemsbergen, M. B. Barnes, M. Bell and M. J. McLaughlin, “Limitations of Soil Microbial Biomass Carbon as an Indicator of soil Pollution in the Field,” Soil Biology and Biochemistry, Vol. 39, No. 10, 2007, pp. 2693-2695. doi:10.1016/j.soilbio.2007.05.014
[2] E. A. Stockdale and P. C. Brookes, “Detection and Quantification of the Soil Microbial Biomass-Impacts on the Management of Agricultural Soils,” The Journal of Agriculture Sciences, Vol. 144, No. 4, 2006, pp. 285-302. doi:10.1017/S0021859606006228
[3] A. Winding, K. Hund-Rinke and M. Rutgers, “The use of Microorganisms in Ecological Soil Classification and Assessment Concepts,” Ecotoxicology and Environmental Safety, Vol. 62, No. 2, 2005, pp. 230-248.
[4] M. Schloter, O. Dilly and J. C. Munch, “Indicators for Evaluating Soil Quality,” Agriculture, Ecosystems and Environment, Vol. 98, No. 1-3, 2003, pp. 255-262. doi:10.1016/S0167-8809(03)00085-9
[5] M. R. Carter, E. G. Gregorich, D. A. Angers, M. H. Beare, G. P. Sparling, D. A. Wardle and R. P. Voroney, “Interpretation of Microbial Biomass Measurements for soil Quality Assessment in Humid Temperate Regions,” Ca- nadian Journal of Soil Science, Vol. 79, No. 4, 1999, pp. 507-520. doi:10.4141/S99-012
[6] L. Ranjard, F. Poly and S. Nazaret, “Monitoring complex Bacterial Communities Using Culture-Independent Molecular Techniques: Application to Soil Environment,” Research in Microbiology, Vol. 151, No. 3, 2000, pp. 167- 177. doi:10.1016/S0923-2508(00)00136-4
[7] A. Wolińska and Z. St?pniewska, “Microorganisms Abundance and Dehydrogenase Activity as a Consequence of Soil Reoxidation Process,” In: M. Miransari, Ed., Soil Tillage and Microbial Activities, Research Singpost Publisher, Kerala, 2011, pp. 111-143.
[8] R. Daniel, “The Metagenomics of Soil,” Nature Reviews Microbiology, Vol. 3, No. 6, 2005, pp. 470-478.
[9] A. Wolińska and R. P. Bennicelli, “Dehydrogenase Activity Response to Soil Reoxidation Process Described as Varied Conditions of Water Potential, Air Porosity and Oxygen Availability,” Polish Journal of Environmental Studies, Vol. 19, No. 3, 2010, pp. 651-657.
[10] J. Gliński, W. St?pniewski, Z. St?pniewska, T. W?odarczyk and M. Brzezińska, “Characteristics of Aeration Properties of Selected Soil Profiles from Central Europe,” International Agrophysics, Vol. 14, No. 1, 2000, pp. 17-31.
[11] W. St?pniewski, Z. St?pniewska, J. Gliński, M. Brzezińska, T. W?odarczyk, G. Przywara, G. Varallay and K. Rajkai, “Dehydrogenase Activity of Some Hungarian Soils as Related to Their Water and Aeration Status,” International Agrophysics, Vol. 14, No. 3, 2000, pp. 341-354.
[12] G. L. Hutchinson, “Biosphere-Atmosphere Exchange of Gaseous N Oxides,” In: R. Lal, J. Kimble, E. Levine and B. A. Steward, Eds., Soil and Global Change, CRC Lewis Publisher, Boca Raton, 1995, pp. 219-236.
[13] M. Brzezińska, T. W?odarczyk, W. St?pniewski and G. Przywara, “Soil Aeration Status and Catalase Activity,” Acta Agrophysica, Vol. 5, No. 3, 2005, pp. 555-565.
[14] J. Zwoliński, “Microbial Biomass versus Soil Fertility in Forest Sites,” Polish Journal of Ecology, Vol. 52, No. 4, 2004, pp. 5553-5561.
[15] Z. Z. Jin, J. Q. Lei, X. W. Xu, J. L. Fan, S. F. Zhao, H. W. Zhou and F. Gu, “Relationships of Soil Microbial Biomass with Soil Environmental Factors in Tarim Desert Highway Shelter-Forest,” in Chinese, Chinese Journal of Applied Ecology, Vol. 20, No. 1, 2009, pp. 51-57.
[16] L. F. Pires, O. O. S. Bacchi and K. Reichardt, “Soil Water Retention Curve Determined by Gamma-Ray Beam Attenuation,” Soil and Tillage Research, Vol. 82, No. 1, 2005, pp. 89-97. doi:10.1016/j.still.2004.06.003
[17] M. A. Malicki and A. Bieganowski, “Chronovoltammetric Determination of Oxygen Flux Density in the Soil,” International Agrophysics, Vol. 13, No. 3, 1999, pp. 273- 281.
[18] R. G. Joergensen, “The Fumigation-Extraction Method to Estimate Soil Microbial Biomass: Calibration of the kEN Value,” Soil Biology and Biochemistry, Vol. 28, No. 1, 1996, pp. 25-31. doi:10.1016/0038-0717(95)00101-8
[19] R. Walczak, B. Witkowska-Walczak and C. S?awiński, “Water Retention and Conductivity of Polish Mollic Gleysols,” in Polish, Acta Agrophysica, Vol. 53, No. 2, 2001, pp. 211- 223.
[20] J. Brouver and H. Anderson, “Water Holding Capacity of Ironstone Gravel in a Typical Plinthoxeralf in Southeast Australia,” Soil Science Society of America Journal, Vol. 64, No. 5, 2000, pp. 1603-1608. doi:10.2136/sssaj2000.6451603x
[21] R. Walczak, E. Rovdan and B. Witkowska-Walczak, “Water Retention Characteristics of Peat and Sand Mixtures,” International Agrophysics, Vol. 16, No. 2, 2002, pp. 161- 165.
[22] T. W?odarczyk, W. St?pniewski, M. Brzezińska and G. Przywara, “Impact of Different Aeration Conditions on the Content of Extractable Nutrients in Soil,” International Agrophysics, Vol. 22, No. 4, 2008, pp. 371-375.
[23] Z. St?pniewska, A. Wolińska and R. P. Bennicelli, “Influence of Soil Water Potential on Microdiffusion of Oxygen in the Eutric Cambisol,” Acta Agrophysica, Vol. 84, No. 1, 2003, pp. 145-152.
[24] A. M. Gajda, “Effect of Different Tillage Systems on Some Microbiological Properties of Soils under Winter Wheat,” International Agrophysics, Vol. 22, No. 1, 2008, pp. 201- 208.
[25] A. M. Gajda, “Microbial Activity and Particulate Organic Matter Content in Soils with Different Tillage System Use,” International Agrophysics, Vol. 24, No. 2, 2010, pp. 129- 137.
[26] N. Jedidi, “Microbial Biomass in a Soil Amended with Different Types of Organic Wastes,” Waste Management Research, Vol. 22, No. 2, 2004, pp. 293-299. doi:10.1177/0734242X04043930
[27] P. Nannipieri, L. Badalucco, L. Landi and G. Pietramellara, “Measurements in Assessing the Risk of Chemicals to the Soil Ecosystem,” In: J. T. Zelikoff, Ed., Ecotoxicology: Responses, Biomarkers and Risk Assessment, SOS Publications, New York, 1997, pp. 507-534.
[28] M. C. Fisk and T. J. Fahey, “Microbial Biomass and Nitrogen Cycling Responses to Fertilization and Litter Removal in Young Northern Hardwood Forests,” Biogeo- chemistry, Vol. 53, No. 2, 2001, pp. 201-223. doi:10.1023/A:1010693614196
[29] K. Mohammadi, “Soil Microbial Activity and Biomass as Influenced by Tillage and Fertilization in Wheat Production,” American-Eurasian Journal of Agriculture and Environmental Sciences, Vol. 10, No. 3, 2011, pp. 330-337.
[30] S. Lerat, L. England, J. Klironomos, P. Pauls, C. Swanton and J. T. Trevors, “Real-Time Polymerase Chain Reaction Detection of the Transgenes for Roundup Ready Corn and Soybean in Soil Samples,” Journal of Agricultural and Food Chemistry, Vol. 53, No. 5, 2005, pp. 1337-1342. doi:10.1021/jf048830+
[31] L. Sagova-Mareckova, L. Cermak, J. Novotna, K. Plhackova, J. Forstova and J. Kopecky, “Innovative Methods for Soil DNA Purification Tested in Soils with Widely Differing Characteristics,” Applied and Environmental Microbiology, Vol. 74, No. 9, 2008, pp. 2902-2907. doi:10.1128/AEM.02161-07
[32] L. I. Singh and P. S. Yadava, “Spatial Distribution of Microbial Biomass in Relation to Land-Use in Subtropical Systems of North-East India,” Tropical Ecology, Vol. 47, No. 1, 2006, pp. 63-70.
[33] E. V. Blagodatskaya, O. S. Khokhlova and S. A. Blagodatskii, “Extractable Microbial DNA Pool and Microbial Activity in Paleosols of Southern Urals,” Microbiology, Vol. 72, No. 6, 2003, pp. 750-755. doi:10.1023/B:MICI.0000008380.57233.9e
[34] M. Hartmann, B. Frey, R. K?lliker and F. Widmer, “Semi-Automated Genetic Analyses of Soil Microbial Communities: Comparison of T-RFLP and RISA Based on Descriptive and Discriminative Statistical Approaches,” Journal of Microbiological Methods, Vol. 61, No. 3, 2005, pp. 349-360. doi:10.1016/j.mimet.2004.12.011

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