Author(s)

Emmanuel Tebandite Kasai¹, François Boemer², Roland Marini Djang’eing’a^3,4, Justin Kadima Ntokumunda^3,5*, Salomon Batina Agasa⁶, Nestor Ngbonda Dauly¹, Jean Pierre Alworong’a Opara¹

¹Department of Pediatrics, Kisangani University Clinics, Faculty of Medicine and Pharmacy, University of Kisangani, Kisangani, DR-Congo.
²Biochemical Genetics Laboratory, Human Genetics, CHU of Liege, University of Liège, Liège, Belgium.
³Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Kisangani, Kisangani, DR-Congo.
⁴Laboratory of Analytical Pharmaceutical Chemistry, Faculty of Medicine, University of Liege, Liege, Belgium.
⁵Department of Pharmacy, School of Medicine and Pharmacy, University of Rwanda, Rwanda.
⁶Department of Internal Medicine, Kisangani University Clinics, Faculty of Medicine and Pharmacy, University of Kisangani, Kisangani, DR-Congo.

emoTypeSC™ test is a new cheap, faster, and appropriate screening method for neonatal diagnosis of sickle cell disease. The literature reports a few cases of its applicability. This study extends the cases study and reviews the available literature. The sample consisted of 99 subjects, including 87 newborns (36 girls and 51 boys; 1.9 - 4.9 kg BW) sampled among 566 babies bone at six hospitals in Kisangani city (Democratic Republic of Congo) during March-April 2019; height infant-adolescents (<18 years); and four adults. Duplicate blood samples of 75 newborns, spotted on filter paper, were transferred to Liège in Belgium for LC-MS test confirmation. Of 99 subjects, 74.74% tested HbAA, 24.26% HbAS and 1% HbSS. The prevalence of HbAS compared to the HbAA phenotype was 15/60 (20%) by HemoTypeSC™ and 14/61 (18.7%) by LC-MS. The concordance between the two methods was 98.3% or a discordance of 1.7%. The findings support the validity of the HemoTypeSC™ test as a sensitive, specific point of care test, cheap and reliable for poor African populations.

Sickle Cell Disease, Hemoglobin Phenotype, HemoTypeSC™, Neonates

Kasai, E. , Boemer, F. , Djang’eing’a, R. , Ntokumunda, J. , Agasa, S. , Dauly, N. and Opara, J. (2020) Systematic Screening of Neonatal Sickle Cell Disease with HemoTypeSC^TM Kit-Test: Case Study and Literature Review. Open Journal of Blood Diseases, 10, 12-21. doi: 10.4236/ojbd.2020.101002.