Author(s)

Daniel E. Meeroff^1*, Bishow Shaha¹, Frederick Bloetscher¹, Nwadiuto Esiobu², Brian Mercer², Donald McCorquordale³, Ramana Kari⁴, Michael Bennett⁵

¹Department of Civil, Environmental & Geomatics Engineering, Florida Atlantic University, Boca Raton, FL, USA.
²Department of Biological Sciences, Florida Atlantic University, Sanson Life Sciences, Boca Raton, FL, USA.
³Applied Biotech Inc., Plantation, FL, USA.
⁴Solid Waste Authority of Palm Beach County, West Palm Beach, FL, USA.
⁵AECOM/URS, West Palm Beach, FL, USA.

Deep-well injection is a cost-effective alternative for industrial wastewater disposal, given the appropriate geology. Fouling of injection well tubing by biofilm or scale is common and reduces the effective diameter of the pipe, which results in increased wellhead pressure and lower injectivity. A detailed microbiological composition of biofilms and abiotic fingerprints use of mineral scale from an injection well has not been reported before. Therefore, biofilm and mineral scale samples from three depth intervals within a deep injection well (surface zone, D1= 0 - 61 m; intermediate zone, D2 = 62 - 457 m; and above the injection zone, D3 = 458 - 884 m) with recurrent biofilm development were collected to characterize the mineral composition and microbial community DNA. X-ray diffraction (XRD) analysis of the solids confirms the composition of the solids collected was mostly calcium carbonate (CaCO₃) with minor contributions from common mineral salts. Microbiological fingerprinting suggests that methanogenic archaea and sulfate-reducing bacteria both of which are anaerobic biofilm producers were the most prevalent members of the prokaryotic community at all sampled depths. Methanosarcinae spp. increased with increasing depths, unlike other archaea. A non-pathogenic biofilm-producing Entamoeba dispar was the most prevalent member of the microbial domain (>30%) in all samples but was highest at the middle depth. The Chao alpha diversity indices for bacteria, viruses, and protozoans were highest at the shallow depth and gradually declined with increasing depth. The prevalent species above the injection zone depth are not barophilic organisms that thrive at high pressures, rather they are sulfate-reducing bacteria involved in anaerobic dissimilatory sulfate metabolism.

Injection Well, Leachate, Industrial Wastewater Disposal, Biofilm Microbiology, Well Rehabilitation, Metagenomic Sequencing

Meeroff, D. , Shaha, B. , Bloetscher, F. , Esiobu, N. , Mercer, B. , McCorquordale, D. , Kari, R. and Bennett, M. (2019) Characterization of Biofilms and Mineralogical Scale in Underground Injection Well Disposal of Landfill Leachate and Industrial Wastewater Streams. Journal of Geoscience and Environment Protection, 7, 69-91. doi: 10.4236/gep.2019.711006.