Quantitative Screening of Secretory Protein Genes in Candidatus Liberibacter Asiaticus - American Journal of Plant Sciences

AJPS > Vol.9 No.12, November 2018

American Journal of Plant Sciences

Volume 9, Issue 12 (November 2018)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.57 Citations

Quantitative Screening of Secretory Protein Genes in Candidatus Liberibacter Asiaticus ()

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DOI: 10.4236/ajps.2018.912174 915 Downloads 10,755 Views Citations

Author(s)

Binbin Li^1,2, Yi Yang³, Zhiwen Luo⁴, Zhixin Liu¹, Naitong Yu^1*

Affiliation(s)

¹Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
²Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
³Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
⁴Tropical Fruit Trees Institute, Hainan Academy of Agricultural Sciences, Haikou, China.

ABSTRACT

Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The disease is caused by Candidatus Liberibacter spp., which is vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Secretory proteins are important in bacterial pathogenesis and structure components. Some of them are expressed at a high level. To obtain the highly-expressed secretory protein genes (SPGs) for antiserum preparation, six candidate SPGs were chosen from Candidatus Liberibacter asiaticus by bioinformatic analysis and were further tested by qPCR and RT-qPCR methods, respectively. The result showed that two SPGs, 408 and pap (both are Flp pilus assembly protein genes), have relative high amounts of DNA and RNA transcripts of early HLB-infected green orange leaves. The 408 and pap genes were further constructed into the plant expression vector pCAMBIA1300 (GV1300: GFP) and expressed in tobacco leaf epidermal cells for subcellular localization analysis. The transient expression results indicated that the 408 protein is located in the nuclei and cytoplasm of tobacco leaf cells. However, the pap protein is located in the cytoplasm of tobacco leaf cells, which may help the pathogen invade into plant cells. This research is an important foundation for the preparation of the antiserum against Candidatus Liberibacter asiaticus and the early detection of HLB disease.

KEYWORDS

Candidatus Liberibacter Asiaticus, Secretory Protein, DNA Amount, RNA Transcription, Subcellular Localization

Share and Cite:

Li, B. , Yang, Y. , Luo, Z. , Liu, Z. and Yu, N. (2018) Quantitative Screening of Secretory Protein Genes in Candidatus Liberibacter Asiaticus. American Journal of Plant Sciences, 9, 2408-2419. doi: 10.4236/ajps.2018.912174.

Cited by

[1]	Unravelling the Pathogenesis and Molecular Interactions of Liberibacter Phytopathogens with Their Psyllid Vectors
	2020

[2]	Construction of CRISPR Plant Expression Vectors Resistant to Papaya Leaf Curl Virus
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[3]	Banana bunchy top virus (BBTV) nuclear shuttle protein interacts and re-distributes BBTV coat protein in Nicotiana benthamiana
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[4]	基于 CRISPR 基因编辑系统抗番木瓜曲叶病毒的植物转化载体的构建
	2019

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