Author(s)

Maria do Carmo Silva Barreto¹, Márcia do Vale Barreto Figueiredo^2*, Márcia Vanusa da Silva¹, Arnóbio Gonçalves de Andrade³, José de Paula de Oliveira², Clébia Maria Alves Almeida¹, Livia Caroline Alexandre de Araújo¹, Odemar Vicente dos Reis Junior⁴, Manoel Urbano Ferreira Junior², Antonio Félix da Costa², Vera Lucia de Menezes Lima^1*

¹Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil.
²Laboratório de Biologia do Solo, Instituto Agronômico de Pernambuco, Recife, Brazil.
³Departamento de Química, Universidade Federal Rural de Pernambuco, Recife, Brazil.
⁴Centro de Tecnologias Estratégicas do Nordeste, Recife, Brazil.

Endophytic bacteria may influence agricultural production in several ways, including promoting plant growth. Two experiments were conducted in order to evaluate the combination of endophytic bacteria from the Brazilian Northeast region aims at the commercial introduction of the inoculation of these bacteria in micropropagated sugarcane plants using a temporary immersion bioreactor. One experiment was done in tubes with sterile commercial substrate, and the other was done in pots with soil; both were installed in a greenhouse. A mixed inoculation was performed in six inoculated endophytic diazotrophic bacteria in micropropagated sugarcane plants, variety RB92579. In the experiment with soil, the mixed inoculation significantly increased the shoot dry matter of plants without the addition of nitrogen fertilizer. However, the accumulation of total-N in the tissues showed no significant differences between treatments with and without nitrogen fertilization. The evaluation of micropropagated seedlings showed no increases in the parameters tested. The results showed that the response of inoculation in temporary immersion bioreactor micropropagation is possible, and that the application of homologous strains may have contributed to a better response by the interaction of endophytic bacteria with sugarcane RB92579. Further studies should be conducted to improve the methodology, which indicates a great potential to optimize this process on a commercial scale.

Biological Nitrogen Fixation, Meristem Culture, Diazotrophic Bacteria, Endophytes, Micropropagation

Barreto, M. , Figueiredo, M. , da Silva, M. , de Andrade, A. , de Oliveira, J. , Almeida, C. , de Araújo, L. , Junior, O. , Junior, M. , da Costa, A. and Lima, V. (2018) Biotechnological Potential of Endophytic Bacteria to Improve the Micropropagated Seedling of Variety RB92579 Sugarcane (Saccharum officinarum L.). Advances in Microbiology, 8, 859-873. doi: 10.4236/aim.2018.811057.