Ex Vivo Characterization of the Action of Sideritis Extract Using Electrical Activity in the Rat Hippocampus Slice Preparation - Pharmacology & Pharmacy

PP > Vol.7 No.10, October 2016

Volume 7, Issue 10 (October 2016)

ISSN Print: 2157-9423 ISSN Online: 2157-9431

Google-based Impact Factor: 0.67 Citations

Ex Vivo Characterization of the Action of Sideritis Extract Using Electrical Activity in the Rat Hippocampus Slice Preparation ()

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DOI: 10.4236/pp.2016.710048 1,354 Downloads 2,655 Views Citations

Author(s)

Wilfried Dimpfel¹, Leonie Schombert², Björn Feistel³

Affiliation(s)

¹Justus-Liebig-University Giessen c/o NeuroCode AG, Wetzlar, Germany.
²NeuroCode AG, Wetzlar, Germany.
³Finzelberg GmbH & Co. KG, Andernach, Germany.

ABSTRACT

A hydroethanolic extract (20% V/V) from Herba Sideritis scardica has been recognized to positively influence cognition. The present investigation aimed at the question if this extract would be able to modify intra-hippocampal communication after oral administration of 100 mg/kg daily for one week. The glutamatergic synapse between Schaffer Collaterals and pyramidal cells can be tested by electric stimulation using single pulses or theta burst stimulation. The resulting population spike is modulated by compounds acting at the central nervous system or other preparations directly or as ex vivo approach. In this case the effect of the special extract was tested in vitro the next day after repetitive in vitro administration. Conventional recording technique in the in vitro hippocampus slice revealed an increase of the population spike in the presence of single stimuli and theta burst stimuli resulting in increased long-term potentiation. This effect was tried to modulate by several glutamate receptor antagonists, among them compounds targeting at the ionic NMDA receptor (CGS19755), AMPA receptor (NBQX), Kainate receptor (UBP301) and targeting at three metabotropic glutamate receptors (mGluR I (YM298198), mGluRII ((RS)-APICA)) and mGluRIII (MSOP). Only NBQX was able to prevent the action of the Sideritis scardica extract. Since the AMPA receptor has been related to cognition in several reports in the literature, it is concluded from this result that the positive action of Sideritis scardica extract on brain function involves a modulation of AMPA receptor dependent neurotransmission.

KEYWORDS

Hippocampus Slice, AMPA Receptor, Sideritis scardica, Greak Mountain Tea, Long Term Potentiation, Hippocampus

Share and Cite:

Dimpfel, W. , Schombert, L. and Feistel, B. (2016) Ex Vivo Characterization of the Action of Sideritis Extract Using Electrical Activity in the Rat Hippocampus Slice Preparation. Pharmacology & Pharmacy, 7, 407-416. doi: 10.4236/pp.2016.710048.

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