e-Business is a vast area of study. Normally Information System researchers preferred to use a combination of quantitative and qualitative study or known as Mixed Method approach. This is because qualitative study is able to explain the quantitative finding in more details. Malaysian e-Supply Chain (e-SC) was chosen as domain for this study and the ultimate goal of research is to identify measurement parameters for trusted trading partner. This study focused on the role of Focus Group Discussion (FGD) as a tool for e-Business research included with the FGD procedure, size of participant, and challenges in conducting the FGD sessions.