Author(s)

A. De la Paz Orozco¹, F. Jiménez Vega¹, S. A. Martel-Estrada², A. Hernández Aguilar³, M. E. Mendoza-Duarte⁴, M. C. Chavarría-Gaytán³, C. A. Rodríguez-González³, I. Olivas-Armendáriz³

¹Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Chihuahua, México.
²Instituto de Arquitectura Diseno y Arte, Universidad Autónoma de Ciudad Juárez, Chih, México.
³Instituto de Ingeniería y Tecnología, Universidad Autónoma de Cd. Juárez, Chihuahua, México.
⁴Centro de Investigación en Materiales Avanzados, Chihuahua, México.

This work focuses to improve the mechanical properties and investigates the growth of osteoblasts on a degradable chitosan/poly(L-lactide)/carbon nanotubes composite for tissue engineering. The morphological and mechanical properties characterizations were performed using scanning electronic microscopy (SEM) and rheometrics analysis system (RSA). Osteoblasts differentiation was determined by alkaline phosphatase activity, total number cells by an Alamar Blue assay, cell attachment and proliferation were visualized qualitatively using SEM, mineralization was characterized by transformed infrared spectroscopy, energy dispersive spectroscopy, and X-ray diffraction. The results suggest that the chitosan/poly(L-lactide)/multiwalled carbon nanotubes composites exhibit the ability to promote cell adhesion, proliferation, and differentiation on their surface.

Chitosan, Poly(L-Lactide), Multiwalled Carbon Nanotubes, Mechanical Properties, Biocompatibility

De la Paz Orozco, A. , Vega, F. , Martel-Estrada, S. , Aguilar, A. , Mendoza-Duarte, M. , Chavarría-Gaytán, M. , Rodríguez-González, C. and Olivas-Armendáriz, I. (2016) Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering. Open Journal of Regenerative Medicine, 5, 14-23. doi: 10.4236/ojrm.2016.51002.