Prevalence of Toxins and Antimicrobial Resistance among E. coli Isolated from Meat - Advances in Microbiology

AiM > Vol.5 No.11, October 2015

Advances in Microbiology

Volume 5, Issue 11 (October 2015)

ISSN Print: 2165-3402 ISSN Online: 2165-3410

Google-based Impact Factor: 1.35 Citations

Prevalence of Toxins and Antimicrobial Resistance among E. coli Isolated from Meat ()

HTML XML

Download as PDF (Size: 846KB) PP. 737-747

DOI: 10.4236/aim.2015.511078 3,293 Downloads 4,781 Views Citations

Author(s)

Shaymaa H. Abdel-Rhman¹, Sara M. Khalifa², Khaled H. Abd El Galil¹, Rasha M. Barwa^1*

Affiliation(s)

¹Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
²Ministry of Health, Mansoura, Egypt.

ABSTRACT

Aim: The present study aims to evaluate the occurrence and characterize E. coli in meat and meat products marketed in Egypt based on their antimicrobial-resistance pattern and production of enterotoxins. Methods: A total of 250 meat samples, categorized as 80 fresh beef, 85 ground beef and 85 beef burger purchased from supermarkets and butchers’ shops were used for isolation of E. coli. All isolates were screened for antimicrobial susceptibility. Plasmid profile analyses were done. Polymerase chain reactions were performed for detection of enterotoxin-encoding genes (astA, eaeA, stx1 and stx2). Results: Twenty-five samples were isolated and identified as E. coli. 14 isolates were multidrug resistant. Plasmids isolation from all isolates revealed that 76% of these isolates harbored plasmids. astA gene was amplified in 7 isolates (28%). Eight (32%) isolates harbored eaeA gene. However, none of the isolates harbored stx1 or stx2 genes. Analysis of multiple drug resistant isolates revealed a significant relation between multiple drug resistance and both astA and eaeA. Conclusion: The study confirmed the prevalence of enterotoxin genes (astA and eaeA) in E. coli isolated from meat product and the association between the presence of these genes and multiple drug resistant phenomena.

KEYWORDS

Enterotoxin, E. coli, Antimicrobial Resistance, Meat Products

Share and Cite:

Abdel-Rhman, S. , Khalifa, S. , Galil, K. and Barwa, R. (2015) Prevalence of Toxins and Antimicrobial Resistance among E. coli Isolated from Meat. Advances in Microbiology, 5, 737-747. doi: 10.4236/aim.2015.511078.

Cited by

[1]	Identifikasi Escherichia coli yang Resistan Antibiotik pada Daging Burger yang Dijual di Sekitar Kampus IPB Dramaga Bogor
	Jurnal Veteriner Desember, 2021

[2]	Frequency of extended spectrum beta lactamase producing Escherichia coli in fresh and frozen meat.
	2021

[3]	Identification of antibiotic resistant Escherichia coli on burger meats sold around IPB University at Dramaga Bogor.
	2021

[4]	Identifikasi Escherichia coli yang Resistan Antibiotik pada Daging Burger yang Dijual di Sekitar Kampus IPB Dramaga Bogor.
	Jurnal …, 2021

[5]	Frequency of Extended Spectrum Beta Lactamase Producing Escherichia coli in Fresh and Frozen Meat
	2020

[6]	Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Edible Offal in Korea
	2019

[7]	Virulence Characteristics, Serotyping and Phylogenetic Typing of Clinical and Environmental Escherichia coli Isolates
	2018

Journals Menu

Follow SCIRP

	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies