Author(s)

Toshiyuki Shibata^1,2*, Kohki Nagayama^2,3, Shingo Sugiura^1,2, Saki Makino^1,2, Mitsuyoshi Ueda^2,4, Yutaka Tamaru¹

¹Graduate School of Bioresources, Mie University, Mie, Japan.
²Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Tokyo, Japan.
³Kumamoto Prefectural Fisheries Research Center, Kumamoto, Japan.
⁴Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

The composition and antioxidative properties of crude phlorotannins isolated from Japanese Eisenia and Ecklonia species including cultured E. kurome were analyzed. As a result of liquid chromatography mass spectrometry analysis, it was suggested that the phlorotannins were composed of compounds containing 3 - 7 phloroglucinol units for Eisenia species and 3 - 8 phloroglucinol units for Ecklonia species. The antioxidant properties of phlorotannins were evaluated using the hydrophilic oxygen radical absorbance capacity (H-ORAC) assay, the measurements of superoxide anion radical scavenging activity (SOSA) with electron spin resonance method, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assay. It was clarified that the phlorotannins contained H-ORAC values of 8.9 × 103 - 1.0 × 104 μmol-Trolox equivalents (TE)/g, SOSA of 1.5 × 104 - 2.9 × 104 superoxide dismutase units/g, DPPH radical scavenging activity of 4.7 × 103 - 5.5 × 103 μmol-TE/g, and reducing power of 6.5 × 102 - 7.0 × 102 mg-ascorbic acid equivalents/g, and had excellent antioxidative properties.

Eisenia Species, Ecklonia Species, Cultured E. kurome, H-ORAC, Phlorotannins, Radical Scavenging Activities

Shibata, T. , Nagayama, K. , Sugiura, S. , Makino, S. , Ueda, M. and Tamaru, Y. (2015) Analysis on Composition and Antioxidative Properties of Phlorotannins Isolated from Japanese Eisenia and Ecklonia Species. American Journal of Plant Sciences, 6, 2510-2521. doi: 10.4236/ajps.2015.615253.