Author(s)

Karim Bouterfas^1*, Zoheir Mehdadi¹, Djamel Benmansour², Meghit Boumedien Khaled³, Mohamed Bouterfas⁴, Ali Latreche¹

¹Laboratory of Vegetal Biodiversity: Conservation and Valorization, Faculty of Life and Natural Sciences, Djillali Liabes University, Sidi Bel-Abbes, Algeria.
²Laboratory of Statistics and Random Model, Faculty of Natural and Universe Sciences, Abou-Bekr Belkaid University, Tlemcen, Algeria.
³Department of Biology, Faculty of Natural and Life Sciences, Université Djillali Liabes, Sidi Bel Abbes, Algeria.
⁴Laboratory of Microscopy, Microanalysis of the Matter and Molecular Spectroscopy, Faculty of Exact Sciences, Djillali Liabes University, Sidi Bel-Abbes, Algeria.

This research was aimed to optimize the extraction conditions of three phenolic compounds: total phenolics, flavonoids and condensed tannins, from White Horehound’s leaves (Marrubium vulgare L.). Distilled water and different organic solvents such as: methanol, ethanol and acetone, were used, with various concentrations (20% - 80%, v/v), temperatures (20°C - 60°C) and extraction times (30 - 450 min). Results showed that the maximum total phenolics amounts (293.34 ± 14.60 mg gallic acid equivalent/g dry weigh), were obtained with 60% aqueous methanol at 25°C for 180 min; total flavonoids (79.52 ± 0.55 mg catechin equivalent/g dry weigh) with 80% aqueous methanol at 20°C for 450 min, and condensed tannins (28.15 ± 0.80 mg catechin equivalent/g dry weigh) with 60% aqueous acetone at 50°C and for 180 min. ANOVA test showed the significant effect (***P < 0.001) of the extraction conditions tested on phenolic compounds. The Principal Component Analysis (PCA) exhibited the positive effect of low temperatures on total phenolics and flavonoids extraction, and the effect of high temperatures on the condensed tannins extraction. The Response Surface Methodology (RSM) provided predicted values of extraction conditions and maximum polyphenols amounts similar to those obtained experimentally.

Marrubium vulgare, Phenolic Compounds, Optimization, Extraction Conditions

Bouterfas, K. , Mehdadi, Z. , Benmansour, D. , Khaled, M. , Bouterfas, M. and Latreche, A. (2014) Optimization of Extraction Conditions of Some Phenolic Compounds from White Horehound (Marrubium vulgare L.) Leaves. International Journal of Organic Chemistry, 4, 292-308. doi: 10.4236/ijoc.2014.45032.