Author(s)

Nikolay L. Lavrik, Nikolay M. Bazhin

Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russian Federation.

A study is made on the previously ignored problem of the dependence of a static fluorescence quenching Stern-Volmer constant K_sv on the initial concentration of [F]₀ fluorophore F. This correlation is shown to exist. It is concluded that the Stern-Volmer quenching constant may be used as association constant K only with .

Fluorescence Quenching, Association Constants, Stern-Volmer Constant

Lavrik, N. and Bazhin, N. (2014) On the Question of Defining the Association Constants by the Method of Fluorescence Quenching. American Journal of Analytical Chemistry, 5, 1065-1068. doi: 10.4236/ajac.2014.516113.