Stool as Appropriate Sample for the Diagnosis of Mycobacterium tuberculosis by Gene Xpert Test - Open Journal of Respiratory Diseases

OJRD > Vol.4 No.3, August 2014

Open Journal of Respiratory Diseases

Volume 4, Issue 3 (August 2014)

ISSN Print: 2163-940X ISSN Online: 2163-9418

Google-based Impact Factor: 0.61 Citations

Stool as Appropriate Sample for the Diagnosis of Mycobacterium tuberculosis by Gene Xpert Test ()

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DOI: 10.4236/ojrd.2014.43012 5,718 Downloads 9,649 Views Citations

Author(s)

Seble Haile Welday, Andrew Nyerere Kimang’a, Beatrice Muthoni Kabera, Josphine Wahogo Mburu, Christina Mwachari, Elizabeth Mungai, Stanley Mugambi Ndwiga, Joseph Kariuki Mbuthia, Gunturu Revathi

Affiliation(s)

Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
Department of Pathology, Aga Khan University Hospital, Nairobi, Kenya.
Gertrude Children’s Hospital, Nairobi, Kenya.
Kiambu District Hospital, Kiambu, Kenya.
National Reference TB Laboratory, Nairobi, Kenya.
University of Maryland Program, Nairobi, Kenya.

ABSTRACT

Background: Diagnosis of pediatric pulmonary tuberculosis (PTB) is a challenge. Symptoms are nonspecific. Young children are unable to expectorate sputum samples; the procedures for obtaining respiratory samples are invasive. Thus Mycobacterium tuberculosis cultures and smears often are not performed. Stool samples were used as an alternative to respiratory samples for the diagnosis of pediatric PTB using stool Xpert MTB/RIF and its sensitivity for detecting the DNA of MTB in stool was determined. Methods: The study was a laboratory-based cross-sectional prospective design. Stool specimen was collected from PTB suspected children (<15 years) attended in Gertrude’s Children’s Hospital Nairobi and Kiambu District Hospital from September 2013 to March 2014. Stool for Xpert was processed in two ways, direct and prior extraction of DNA using QIAGEN stool DNA extraction kit. Result: A total sample of 91 stool specimen was collected from patients. Of these 53 (58.2%) had sputum ZN smear microscopy. Six (11.3%) of them were confirmed smear positive for PTB. Stool Gene Xpert was positive in all the six smear positive children. Four (7.5%) smear negative patients tested positive by stool Gene Xpert test. This association is significant (P = 0.000). Conclusion: This study reports that Mycobacterium tuberculosis DNA can be detected in stool using Xpert testing with a higher sensitivity. Therefore stool which can easily be obtained is an appropriate alternative sample for the diagnosis of PTB using Xpert assay for children unable to give respiratory samples. Furthermore Xpert turn round time is less than 2 hours.

KEYWORDS

Pulmonary Tuberculosis (PTB), Pediatric, Stool, Xpert MTB/RIF, Smear Microscopy, Mycobacterium tuberculosis

Share and Cite:

Welday, S. , Kimang’a, A. , Kabera, B. , Mburu, J. , Mwachari, C. , Mungai, E. , Ndwiga, S. , Mbuthia, J. and Revathi, G. (2014) Stool as Appropriate Sample for the Diagnosis of Mycobacterium tuberculosis by Gene Xpert Test. Open Journal of Respiratory Diseases, 4, 83-89. doi: 10.4236/ojrd.2014.43012.

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