Author(s)

Sana Khan, Marie Olszewski, Wei Huang, Morris Kletzel

Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA.
Stem Cell Transplant Chimerism Laboratory, Depts. of Hema-tology-Oncology Transplant; Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, USA; Northwestern University Feinberg School of Medicine, Chicago, USA.

This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71⁺CD34⁺ using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71⁺CD34⁺ in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.

Juvenile Myelo-Monocytic Leukemia; Wilms Tumor; Nested PCR; JMML; WT1; GM-CSF; nPCR

Khan, S. , Olszewski, M. , Huang, W. and Kletzel, M. (2014) Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (WT1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry. Advances in Bioscience and Biotechnology, 5, 155-159. doi: 10.4236/abb.2014.52019.