Dynamic capacity increase in high voltage electric power transmission line is currently the most economical method for solving electric power transmission bottleneck nowadays. DS18B20 temperature sensor is applied to the dynamic capacity increase of high voltage transmission lines to measure the conductor temperature and ambient temperature. The paper is focused on the experiment of DS18B20 both in the laboratory and outside. From the result of the lab temperature measurement data analysis, using 4 DS18B20’s is the most suitable plan, considering both accuracy and economical efficiency. In the experiment outside, we get four groups of conductor (uncharged) temperature and four groups of ambient temperature. The data proved that DS18B20 has good stability, and small measurement error. It is suitable for measuring the temperature of conductor and ambient in dynamic capacity increase, and helpful to improve the accuracy of the calculation of capacity increasing.