Objective: Evaluate the effect of a physical activity program on the blood lipid profile in women between 40 and 55 years old. Methods: 7 female participants from the city of Chihuahua, Chihuahua, Mexico, on a program of moderateintensity physical activity lasting 4 months, evaluated before and after the implementation of the program in the variables of total cholesterol, triglycerides, LDL and HDL, as well as the atherogenic index and cardiovascular risk. Data were analyzed with Student t test for related samples with a significance level of 0.05. Results: Total cholesterol levels and LDL in samples collected after physical exercise program decreased significantly compared to those collected before the implementation of the exercise program, while statistically, HDL and triglycerides did not show significant changes. The atherogenic index and cardiovascular risk showed positive trends, thus favoring that moderate intensity aerobic exercise decreased cardiovascular risk in women reaching the perimenopause period.