American Journal of Plant Sciences

Volume 3, Issue 12 (December 2012)

ISSN Print: 2158-2742   ISSN Online: 2158-2750

Google-based Impact Factor: 1.57  Citations  

Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination

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DOI: 10.4236/ajps.2012.312202    12,630 Downloads   20,782 Views  Citations

ABSTRACT

Chemical and physical mutagenesis has been used to increase genetic variability in crop plants. More than 430 new varieties have been derived as mutants of rice (Oryza sativa L.) via the application of different mutagenic agents. Chemical mutagens such as ethyl methane sulphonate (EMS), diepoxybutane-derived (DEB), sodium azide and irradiation (Gamma rays, X-rays and fast neutrons) have been widely used to induce a large number of functional variations in rice and others crops. Among chemical mutagens, the alkylating agent, ethyl methane sulfonate (EMS) is the most commonly used in plants as it causes a high frequency of nucleotide substitutions, as detected in different genomes. In this study, seeds of potential genotype of the popular variety, (Oryza sativa L. spp. Indica cv. MR219) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1%, 1.25%, 1.5% and 2%. Sensitivity to EMS was determined by various measurements on the M1 generation. As concentration of applied EMS increased, will decrease in germination, seedling height, root length and emergence under field conditions was observed in M1 generation as compared to the non-treatment control. Plant height and root length also decreased with increases in EMS mutagenesis in an approximately linear fashion. The LD25 and LD50 values were observed based on growth reduction of seedlings after EMS treatment with 0.25% and 0.50% on the rice variety (Oryza sativa L. spp. Indica cv. MR219).

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Talebi, A. , Talebi, A. and Shahrokhifar, B. (2012) Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination. American Journal of Plant Sciences, 3, 1661-1665. doi: 10.4236/ajps.2012.312202.

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[184] Ethyl Methanesulfonate Mutagenesis and In Vitro Polyethylene Glycol Selection for Drought Tolerance in Sugarcane (Saccharum spp.)
Sugar Tech, 2017
[185] Determination of the optimal condition for ethylmethane sulfonate-mediated mutagenesis in a Korean commercial rice, Japonica cv. Dongjin
Applied Biological Chemistry, 2017
[186] Single Nucleotide Polymorphisms in B-Genome Specific UDP-Glucosyl Transferases Associated with Fusarium Head Blight Resistance and Reduced …
2017
[187] Pengaruh Mutagen Kimia EMS (Ethyl Methane Sulphonate) Terhadap Daya Berkecambah Benih Tanaman Tembakau var. Marakot
2017
[188] Наблюдения върху развитието и разпространението на заболяването черно гниене с причинител Phyllosticta ampelicida (безполов стадий от Guignardia bidwellii) при биологично и конвенционално земеделие в условията на 2015 и 2016 година
2017
[189] Engineered Nickel Oxide Nanoparticle Causes Substantial Physicochemical Perturbation in Plants
Frontiers in Chemistry, 2017
[190] Studies on mutagenic effects of gamma radiation and ethyl methane sulphonate on qualitative and quantitative traits in Tagetes patula L.(French Marigold)
Thesis, 2017
[191] Cytological Effects of Mutagenic Agents and NaCl on Mitotic Division in Two Iraqi Rice (Oryza sativa L.) Genotypes
2017
[192] Частота и спектр мутаций у растений льна (Linum humile Mill.) под действием новых производных диметилсульфата
2017
[193] Индукция на мутантен фенотип в М1 поколение на Paulownia tomentosa, чрез третиране с етил метан сулфонат
2017
[194] Đánh giá và chọn lọc các dòng mía (Saccharum officinarum L.) chịu mặn tái sinh từ mô sẹo đã được xử lý ethyl methane
2016
[195] Identificação de variabilidade para caracteres de importância agronômica em famílias mutantes de arroz irrigado
2016
[196] EMS 诱导辣椒 “6421” 突变体库的构建及矮秆突变体的鉴定与分析
农业科学与技术: 英文版, 2016
[197] STUDIES ON CHARACTERIZATION OF HYPER PHOSPHATE SOLUBILIZING MUTANT STRAIN OF Bacillus licheniformis
Thesis, 2016
[198] Production de génotypes mutants chez le blédur (Triticum durum) : une source de variabilitégénétique pour la tolérance à la sécheresse
2016
[199] Construction of Mutant Population and Analysis of Dwarf Mutants in" 6421"(Capsicum annuum L.) through EMS Mutagenesis
Agricultural Science & Technology, 2016
[200] Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF)
British Microbiology Research Journal, 2016
[201] Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and InVitro Selection in the Low pH Media
HAYATI Journal of Biosciences, 2016
[202] Development of improved soybean (Glycine max, L. Merill) germplasm through induced and targeted mutagenesis
ProQuest Dissertations Publishing, 2016
[203] Exploration of Viability of Ketorolac on Isolated Leydig Cells of Bucks (Capra hircus) In Vitro: Part I
2016
[204] Ethyl methane sulphonate (EMS) mediated changes in callus growth of clusterbean ('Cyamopsis tetragonoloba'L.) raised under saline conditions
2016
[205] Callus Induction and Proliferation as Affected by EMS and UV-B Mutagens in Rice (Oryza sativa L.) Genotypes
Iraqi Journal of Science, 2016
[206] Identification of variability for agronomically important traits in rice mutant families
Bragantia, 2016
[207] Effect of EMS on germination and survival of okra (Abelmoschus esculentus L.)
BIHAREAN BIOLOGIST, 2016
[208] Determination of LD50 of ethyl methanesulfonate (EMS) for induction of mutations in rapeseed-mustard
2016
[209] EMS 诱导辣椒 “6421” 突变体库的构建及矮秆突变体的鉴定与分析
农业科学与技术: 英文版, 2016
[210] FIELD EVALUATION OF BURLEY LINES CONTAINING ALLELES MINIMIZING NICOTINE TO NORNICOTINE CONVERSION
2016
[211] ETHYL METHANE SULFONATE AND ITS EFECT ON MUTAGENESIS IN RICE CROP IMPROVEMENT
2016
[212] Mutasi Induksi Kimia Pada Coleus Spp. Dengan Ethyl Methane Sulphonate (Ems)
Dissertations and Theses, 2016
[213] Determination of LD
2016
[214] Ethyl Methanesulphonate (EMS) Induced Mutagenic Disorders in Amaranthus tricolor L.
International Journal of Science and Research, 2016
[215] FAKULTAS PERTANIAN UNIVERSITAS JEMBER 2016
2016
[216] Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase …
2016
[217] Ethyl methane sulfonate and its effect on mutagenesis in rice crop improvement
2016
[218] Ethyl Methanesulphonate (EMS) Induced Mutagenic Disorders in Amaranthus tricolor L
2016
[219] Strain development of Aspergillus brasiliensis using physical and chemicals mutagenesis for possible overproduction of xylanase, amylase, protease and …
British Microbiology Research Journal, 2016
[220] Investigation of EMS effect on Vicia faba seed germination and seedling growth under saline condition
2016
[221] Effect of ethyl methanesulfonate (EMS) mutagen on genetic variability, growth characters and yield of potato
2015
[222] Assessment of EMS-induced variations in rice mutant lines using ISSR marker
2015
[223] 11. STUDIES ON MUTAGENIC EFFECTS OF GAMMA RAYS AND ETHYL METHYL SULFONATE ON M 1 GENERATION IN ADT 47 RICE By D. RAJARAJAN 1 _ R …
2015
[224] STRAIN IMPROVEMENT Acetobacter xylinum MENGGUNAKAN ETHYL METHANE SULFONATE (EMS) SEBAGAI UPAYA PENINGKATAN PRODUKSI SELULOSA …
2015
[225] KARAKTER MOLEKULER KEDELAI (Glycine max) TOLERAN KEKERINGAN HASIL INDUKSI MUTASI DENGAN MUTAGEN EMS (ETHYL METHANE SULFONATE)
2015
[226] Molecular characterization of GIANT CHLOROPLAST in rice (Oryza sativa L.) involved in chloroplast division
2015
[227] Mutation Breeding and Salt Stress Tolerance in Plants
Managing Salt Tolerance in Plants: Molecular and Genomic Perspectives, 2015
[228] STRAIN IMPROVEMENT Acetobacter xylinum MENGGUNAKAN ETHYL METHANE SULFONATE (EMS) SEBAGAI UPAYA PENINGKATAN PRODUKSI SELULOSA BAKTERI [IN PRESS JANUARI 2016]
Jurnal Pangan dan Agroindustri, 2015
[229] Ethyl methane sulphonate induced genetic variability and heritability in macrosperma and microsperma lentils
Journal of Environmental Biology, 2015
[230] Generation of chemically induced mutations using in vitro propagated shoot tip tissues for genetic improvement of fruit trees
Plant Cell, Tissue and Organ Culture (PCTOC), 2015
[231] Ethyl methane sulfonate induced mutations in M2 generation and physiological variations in M1 generation of peppers (Capsicum annuum L.)
Frontiers in plant science, 2015
[232] Non-targeted mutagenesis of Ornithogalum candicans through exposure to ethyl methanesulfonate©
2015
[233] Effect of Proton Beam Irradiation on M1 Seeds and Seedling Growth in Rice
Plant Breeding and Biotechnology, 2015
[234] Desenvolvemento e caracterización dunha poboación de mutantes de xudía común (Phaseolus vulgaris L.)
2015
[235] STUDIES ON MUTAGENIC EFFECTS OF GAMMA RAYS AND ETHYL METHYL SULFONATE ON M 1 GENERATION IN ADT 47 RICE
2015
[236] Sprout differentiation and mutation induction of garlic (Allium sativum L.) callus exposed to gamma radiation
2015
[237] 长春密刺黄瓜突变体库的构建和部分性状分析
2015
[238] Содержание этерифицированных жирных кислот в семенах мутантных линий ярового рапса (Brassica napus L.)
2014
[239] EMS-induced mutagenesis and DNA polymorphism assessment through ISSR markers in Neolamarckia cadamba (kelampayan) and Leucaena leucocephala …
2014
[240] 鳳梨葉緣刺遺傳與台農 4 號鳳梨誘變育種之研究
2014
[241] GENETIC STUDIES IN CASSAVA (Manihot esculenta Crantz.) GERMPLASM AND ISOLATION OF MUTANTS WITH ALTERED STARCH FOR AUGMENTING …
Thesis, 2014
[242] Studies on efficiency and effectiveness of EMS for induction of mutation in pigeonpea (Cajanus cajan (L.) Millsp.)
Thesis, 2014
[243] The reduction of mimosine content in Leucaena leucocephala (petai belalang) leaves using ethyl methanesulphonate (EMS).
Archives of Applied Science Research, 2014
[244] EMS-induced mutagenesis and DNA polymorphism assessment through ISSR markers in Neolamarckia cadamba (kelampayan) and Leucaena leucocephala (petai belalang)
European Journal of Experimental Biology, 2014
[245] EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine
Plant cell reports, 2014
[246] Kill curve analysis and response of first generation Capsicum annuum L. B12 cultivar to ethyl methane sulfonate.
Genetics and molecular research: GMR, 2014
[247] Ethyl methane sulfonate induces disease resistance in Begonia× hiemalis Fotsch.
Horticulture, Environment, and Biotechnology, 2014
[248] Determination of Lethal Dose and Effect of Ethyl Methane Sulphonate in Rice Varieties
Trends in Biosciences, 2014
[249] Biotechnological Optimization of Light Use Efficiency in Nannochloropsis Cultures for Biodiesel Production
2014
[250] Genetic studies in cassava manihot esculenta crantz Germplasm and isolation of mutants with altered starch for augmenting industrial value
2014
[251] 9. FIXATION OF LETHAL DOSE AND EFFECT OF ETHYL METHANE SULFONATE INDUCED MUTAGENESIS IN RICE ADT (R) 47 By D. RAJARAJAN1_ R …
2014
[252] Determinación de la DL50 de Metanosulfonato de Etilo (EMS) para la inducción de cambios morfológicos y fisiológicos en plántulas de Plukenetia volubilis …
[253] Pengaruh Mutagen Kimia Ethyl Methane Sulfonate (EMS) terhadap Pertumbuhan Tanaman Anggrek Dendrobium sp. Secara In Vitro
[254] Mutation Breeding Strategies for High Energy Tree Species Prosopis juliflora in Pakistan
[255] Analyzing frequency and spectrum of chlorophyll mutation induced through Gamma ray and Combination treatment (Gamma+ EMS) on genetic paradigm …
G Kumar
[256] Genetic variability investigation of Capsicum frutescens var. Cakra Hijau induced by ethyl methane sulphonate (EMS) using SSR DNA markers
[257] Determination of the Effects of Different EMS Doses Applied to Seeds of Chickpea and Lentil Varieties on Some Seedling Characteristics
[258] Phenotypic Characterization of Male Sterile Mutants Induced by Gamma-Rays for Improved Agronomic Traits and Breeding Potential of African Nightshade (Solanum …
[259] Induced Mutation  Affect Seed Germination, Plant Survival and Phenotypic Characteristics Of African Nightshade ( Solanum Villosum)
[260] Studies on effectiveness, efficiency of gamma rays, EMS and their combination in M2 generation of Him Palam Hara Soya 1 variety of soybean [Glycine max (L.) …
[261] This is what you need to know about genetics and its application in breeding our food crops and animals

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