Author(s)

Juthaporn Saengprajak, Piyaporn Saensouk

Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, Thailand..

DNA polymorphism in the cultivar species; Cucumis sativus L., C. melo L. and Benincasa hispida Cogn. of subtribe Cucumerinae (Cucurbitaceae) in the four northeastern provinces of Thailand was examined by using RAPD technique. Twenty 10-mer primers were produced 212 RAPD fragments, ranging from approximately 120 to 2531 bp. The genetic similarities were estimated from banding profiles using a NTSYS* version 2.1 as a basis for dendrogram construction via the UPGMA method. Cluster analysis divided the taxa under study into 2 clades. Moreover, a RAPD marker: Cm (OPJ11₇₀₀) was specified to C. melo, and this marker was converted into sequence characterized amplified region (SCAR) marker: Cm (SCJ11₅₁₆). A pair of sequence-specific primer of clones Cm (OPJ11₇₀₀) amplified a distinct single band of the same size as the RAPD clones. The SCAR marker was developed successfully to identify C. melo genotype.

Cucumerinae; Cucurbitaceae; Genetic Diversity; Random Amplified Polymorphic DNA (RAPD); Sequence Characterized Amplified Region (SCAR); Species Identification

Saengprajak, J. and Saensouk, P. (2012) Genetic Diversity and Species Identification of Cultivar Species in Subtribe Cucumerinae (Cucurbitaceae) Using RAPD and SCAR Markers. American Journal of Plant Sciences, 3, 1092-1097. doi: 10.4236/ajps.2012.38131.