Author(s)

Papa Adama Ndione^1,2, Coumba Gueye¹, Moussa Mbaye¹, Latyr Ndione¹, Khémesse Kital¹, Olivier Maurice Ali Mbaye¹, Lamine Cissé¹, Serigne Omar Sarr², Djibril Fall², François Delattre³, Atanasse Coly¹, Mame Diabou Gaye-Seye¹, Alphonse Tine¹

¹Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
²Laboratoire National de Contrôle des Médicaments, Dakar, Sénégal.
³Laboratoire de Synthèse Organique et Environnement (LSOE, EA2599), Université du Littoral Côte d’Opale, Dunkerque, France.

In this article, we propose developing a reliable and sensitive method for determining tryptamine (TRYP) levels in canned fish samples using spectrofluorimetric analysis. The analytical performance obtained was satisfactory, with detection limits (LOD) ranging from 0.09 ng/mL to 1.16 ng/mL and quantification limits (LOQ) from 0.30 ng/mL to 3.87 ng/mL. Additionally, low values of relative standard deviation (RSD) between 0.56% and 5.56% were achieved, demonstrating a good reproducibility of our measurements. The application of this method to our samples allowed for the detection of tryptamine with satisfactory recovery rates between 94.35% and 107.14%. Finally, the potential interference from biogenic amines, which may be present in fish, was studied. Our results showed that the spectrofluorimetric method is simple, rapid and sensitive enough for routine analysis, requiring neither expensive equipment nor tedious chemical pre-treatments.

Tryptamine, Analysis, Fish, Levels, Fluorescence

Ndione, P. , Gueye, C. , Mbaye, M. , Ndione, L. , Kital, K. , Mbaye, O. , Cissé, L. , Sarr, S. , Fall, D. , Delattre, F. , Coly, A. , Gaye-Seye, M. and Tine, A. (2025) Determination of Tryptamine Level by Spectrofluorimetric Method: Optimization and Application to Canned Fish. American Journal of Analytical Chemistry, 16, 117-133. doi: 10.4236/ajac.2025.166007.