Common Artifacts and Remedies in Histological Preparations ()
ABSTRACT
The importance of the accuracy of preparing biological specimen as
histological sections that can be examined under a microscope lies in reflecting
a true image of the tissue that includes all its components, which are used in
scientific research or for the purpose of diagnosing various diseases of the
body. Despite this, some cellular structures within the tissue may suffer from
some alterations that result from the appearance of defects during any stage of
preparing these microscopic sections, which alter or interfere with the precise
cellular structures and morphology that constitute the tissue and thus give a
different image for tissue features and cause confusion in the work
histopathologist in the diagnosis. There are several reasons that can cause a
misdiagnosis of the sample that occurs during the surgical separation process
or after separation during the stages of microscopic preparation techniques
from fixation stage, tissue processing, embedding or microtomy, staining until
mounting procedures. The constant need to identify these defects and their
causes in addition to try to reduce them is one of the biggest challenges
evident in pathology laboratories. Therefore, this study aims to review the
most common defects that occur in any stage of tissue processing, with an
explanation of their causes and appropriate ways to avoid them.
Share and Cite:
Al-Kinani, M. (2024) Common Artifacts and Remedies in Histological Preparations.
Advances in Bioscience and Biotechnology,
15, 174-183. doi:
10.4236/abb.2024.153012.
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