Author(s)

Magni Pacha Tatiana Flore¹, Tene Tayo Paul Martial^1*, Foka Tatiekam Ebenezer¹, Dzelamonyuy Aristide², Ewane Cecile Annie^1,3, Mbouopda Herman Désire⁴, Boudjeko Thaddée^1,3*

¹Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon.
²Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, USA.
³Department of Biochemistry, University of Yaounde I, Yaounde, Cameroon.
⁴Department of Biology, University of Bamenda, Bamenda, Cameroon.

Three fungicide formulations, namely M1, M2 and M3, were prepared from sodium bicarbonate, citronella essential oil and sunflower slurry. The stability of M1, M2 and M3 formulations was determined based on pH, temperature, order of incorporation of the inputs and storage time. The most stable formulations were used for antagonistic tests on Pythium myriotylum. The Minimum Inhibitory Concentration (MIC) was used for the greenhouse tests and the mode of action was determined in vitro. The study showed that the order of incorporation of the inputs “Essential Oil-Tween 80-Bicarbonate-Slurry” (EO-T80-B-S) promotes stability. M1 and M2 are stable at 4°C, 25°C, 37°C and 40°C temperatures and have a pH of 7 and 8 respectively. The Minimum Inhibitory Concentration of M1 and M2 is 1% on P. myriotylum. M1 and M2 act on Pythium by membrane lysis, inhibiting proton pumps and inhibiting protein synthesis. The formulations M1 and M2 reduce the incidence of root rot disease in cocoyam plants growth in the greenhouse. M1 and M2 are potential candidates for improvement of cocoyam seedlings production in Cameroon.

Biofungicide, Xanthosoma sagittifolium, Pythium myriotylum, Root Rot Disease

Flore, M. , Martial, T. , Ebenezer, F. , Aristide, D. , Annie, E. , Désire, M. and Thaddée, B. (2023) Formulation of Biofungicides from Cymbopogon citratus and Tithonia diversifolia: Evaluating Its Antimicrobial Activities against Pythium myriotylum, the Causal Agent of Root Rot of Xanthosoma sagittifolium (L.) Schott. American Journal of Plant Sciences, 14, 896-914. doi: 10.4236/ajps.2023.148060.