Contrary to the other multi-carrier modulation systems, the coherent optical orthogonal frequency division multiplexing communication system with an offset quadrature amplitude modulation (CO-OFDM-OQAM) possesses inherent imaginary interference (IMI). This has an important impact on the channel estimation process. Currently, a variety of frequency-domain channel estimation methods have been proposed. However, there are various problems that still exist. For instance, in order to reduce the influence of IMI, it is necessary to insert more guard intervals between the training sequence and the payload, leading to the occupation of excessive spectrum resources. In order to address this problem, this work designs a high spectral efficient frequency-domain channel estimation method for the polarization-division-multiplexing CO-OFDM-OQAM systems. First, the working principle of the proposed method is described in detail. Then, its spectral efficiency, power peak-to-average ratio, and channel estimation performance are studied based on simulations. The simulation results show that the proposed method improves the spectral efficiency without worsening the power peak-to-average ratio. The channel estimation capability of this method is verified in three scenarios of long-distance transmissions, including back-to-back, 100 km, and 200 km transmissions.