Author(s)

Xiaoyu Jiang^1*, Lihua Qin^2,3*, Jing Wei^2,3, Guosheng Su^2,3, Xiaoye Su^4,5, Aihua Lu^2,3, Jiansheng Huang^2,3, Feng Lu^2,3, Taixin Lu^2,3, Pinchun Huang^2,3, Buqing Su⁶

¹Department of Laboratory Medicine, Third People’s Hospital of Nanning, Nanning, China.
²Guangxi-ASEAN Economic and Technological Development Zone People’s Hospital, Nanning, China.
³The Department of Nephrology & Laboratory Medicine of Tenth People’s Hospital of Nanning, Nanning, China.
⁴Department of Nursing, Graduate School of Everlasting, University of the Philippines, Fuzhou, China.
⁵Department of Critical Care Medicine, Fuzhou Second People’s Hospital, Fuzhou, China.
⁶Department of Nursing, Liaoning Vocational College of Medicine, Shenyang, China.

Objective: To establish the diagnostic concentration range of urine cystatin C and the control level of urine cystatin C for patients with renal injury, and to help promote the establishment of standardization of urine cystatin C detection. Methods: 150 urine specimens and blood specimens from kidney injury patients and healthy people were collected, and stored in the refrigerator at -80°C for later use. After the specimens were collected, they were uniformly tested. Comparing the difference of blood and urine cystatin C between the kidney injury group and the healthy control group, the application value of urine cystatin C in the diagnosis and treatment of kidney injury was put forward. Results: The concentrations of cystatin C in urine and blood of renal injury group were 1.04 ± 2.14 mg/L and 1.94 ± 2.36 mg/L respectively; the concentrations of cystatin C in urine and blood of healthy control group were 0.11 ± 0.05 mg/L and 0.83 ± 0.20 mg/L respectively. The urine and blood cystatin C of the kidney injury group were significantly higher than those of the healthy control group. Compared the results of the two groups, the t values were 5.3210 and 5.7399, respectively. The P value is 0.0000; the difference is statistically significant; in comparison of urine and blood cystatin C in the kidney injury group, the value is that t = 3.4600, P = 0.0003; in comparison of urine and blood cystatin C in the healthy control group, the value is that t = 42.7744, P = 0.0000. By investigating the urine cystatin C concentration of patients with kidney injury, whether it is kidney injury or healthy controls, urine cystatin C is significantly lower than blood cystatin C. Conclusion: According to the results of this study, the concentration of urinary cystatin C in the healthy control group is 0.11 ± 0.05 mg/L. The reference interval of urinary cystatin C proposed in this study is 0.06 - 0.16 mg/L, which can be established by comprehensive multi center research. Through a multi-center study of the baseline level of urinary cystatin C in patients with renal injury, the diagnostic concentration interval of urinary cystatin C and the control level of urine cystatin C for renal injury patients were established.

Urine, Cystatin C, Kidney Injury, Concentration Interval, Standardization

Jiang, X. , Qin, L. , Wei, J. , Su, G. , Su, X. , Lu, A. , Huang, J. , Lu, F. , Lu, T. , Huang, P. and Su, B. (2022) Urine Cystatin C Determination in the Establishment of Reference Interval in the Diagnosis and Treatment of Renal Injury. Natural Science, 14, 13-17. doi: 10.4236/ns.2022.141002.