Author(s)

Olayinka Anthony Awoyinka^1*, Tolani Rachael², Funmilola Comfort Oladele¹, Margret Olutayo Alese³, Elijah Olalekan Odesanmi⁴, Daisi David Ajayi⁵, Gbenga Sunday Adeleye⁶, Bunmi Comfort Boyede²

¹Department of Medical Biochemistry, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria.
²Department of Microbiology, Ekiti State University, Ado Ekiti, Nigeria.
³Department of Anatomy, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria.
⁴Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria.
⁵Department of Chemical Pathology, Ekiti State University Teaching Hospital, Ado Ekiti, Nigeria.
⁶Department of Physiology, Ekiti State University, Ado Ekiti, Nigeria.

The ubiquitous consumption of junk foods has drastically contributed to the exponential rise in the incidence of obesity. Hence, the present study explores the therapeutic effect of selected indigenous wild bean Sphenostylis stenocarpa (Otili) and condiment fermented Parkia biglobosa (Iru) on obese rats. The rats were fed with a high fat diet for four weeks and the gut microbiota was monitored every other day throughout the period of the experiment. Then, the fecal metabolome was analysed by Gas Chromatography Mass Spectroscopy (GC-MS). Although there was a decrease in the mean weight of rats treated with fermented iru compared with those given Otili, it was not statistically significantly (p ≤ 0.05). The organisms identified from the fecal samples of the fermented Iru groups are Proteus vulgaris, Bacillus cereus and Esherichia coli while those identified from the Otili group include Escherichia coli, and Citrobacter Freundii. However, further study revealed that Otili and Iru had a similar faecal metabolome. Medium chain fatty acids, such as Decanoic acid, Octanoic acid, ethyl tetradecanoate, Hexadecanoic acid, Methyl tetradecanoate, 9-Hexadecanoic acid, Hexadecnoic acid, cis-10-Hepadecanoic acid, are the most common compounds found in this study. This suggests the fact that the associated gut microbiota from breakdown of respective food samples must have actively mediated in their roles of ameliorating the effect of obesity.

Microbiota, Metabolomes, MCFA, SCFA, Obese

Awoyinka, O. , Rachael, T. , Oladele, F. , Alese, M. , Odesanmi, E. , Ajayi, D. , Adeleye, G. and Boyede, B. (2021) Fecal Metabolomes in Response to Feed Supplemented with Fermented Parkia biglobosa and Sphenostylis stenocarpa in Obese Rats. Advances in Microbiology, 11, 63-74. doi: 10.4236/aim.2021.112005.