Author(s)

Steffi Witter^1,2,3, Georg Arju², Marina Junusova², Maria Kuhtinskaja³, Ago Samoson¹, Raiker Witter^4,5, Raivo Vilu²

¹Department of Health Technologies, Tallinn University of Technology, Tallinn, Estonia.
²Competence Center of Food and Fermentation Technology (TFTAK), Tallinn, Estonia.
³Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
⁴Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
⁵Institute for Quantum Optics, Ulm University, Ulm, Germany.

Spectral fluorescence signature, Gas Chromatography-Mass Spectrometry and Liquid Chromatography-Mass Spectrometry for identification of chemical and bioactive compounds were applied to study the plant extracts of Withania somnifera, Centella asiatica and Bacopa monnieri which are related to the possible treatment of mental diseases as Alzheimer, Parkinson and Depression. These plants are known for different positive phytotherapeutic effects on the human brain without negative post-, adverse or after effects to the treated individuals, and have been recommended in several medical studies. Therefore, we selected these plants for further analysis, based on the inhibition results of in vitro Amyloid Beta fibrillation tests made by previous measurements. With this study a first screening of the complex plant extract mixtures was performed, to get an initial overview about known and unknown ingredients. In all three plants, similar main compounds were identified, however in different quality and quantity. These may provide substantial information on which compound combinations might be mainly responsible for the positive effects and should be further investigated being responsible for reducing the fibrillation process of Amyloid Beta.

Gas Chromatography-Mass Spectrometry, Liquid Chromatography-Mass Spectrometry, Principal Component Analysis, Spectra Fluorescence Signature, Extracts, Withania somnifera, Bacopa monnieri, Centella asiatica

Witter, S. , Arju, G. , Junusova, M. , Kuhtinskaja, M. , Samoson, A. , Witter, R. and Vilu, R. (2020) Compound’s Pre-Screening of Withania somnifera, Bacopa monnieri and Centella asiatica Extracts. Journal of Biosciences and Medicines, 8, 80-98. doi: 10.4236/jbm.2020.89007.