Author(s)

José González¹, Max Monan², Julio Perez³, Enrique Gómez⁴, Damaris De La C. Salgado⁴, Danae Pérez⁴

¹Faculty of Technical Sciences, University of Pedagogical Sciences, Havana, Cuba.
²ARVARNAM, Martinica, France.
³National Center of Toxicology, Military Hospital “Dr. Carlos J. Finlay”, Havana, Cuba.
⁴Institute of Pharmacy and Foods, Havana University, Havana, Cuba.

After CC separation of 21 samples from Theobroma cacao Husk was analyzed by GC-MS, from ethanolic extract at 90% was recovered a solid residue of 1 g after several days on the table of the Lab. This main residue (300 mg) was analyzed after successive separations with mixtures of CHCl3: MeOH (7:3) and CHCl3: MeOH (5:5) in CC using Sílica Gel G 60-120 Mesh. All samples were analyzed by TLC on silica gel with fluorescent indicator 254 nm on aluminum cards (layer thickness 0.2 mm) (10 × 20 cm) using CHCl3: MeOH: n-propanol: water (5:6:1:4) as eluent (v/v/v). The presence of Caffeine (CF) and Theobromine (TB) was confirmed after several analyses of the samples by their characteristic fragmentation pattern but nevertheless was found the presence of Theophylline.

Theobroma cacao, Caffeine, Theobromine, GC-MS, CC, TLC

González, J. , Monan, M. , Perez, J. , Gómez, E. , Salgado, D. and Pérez, D. (2019) Determination of Theobromine and Caffeine in Theobroma cacao Husk from Ethanolic Extract by GC-MS after CC Separation. Open Access Library Journal, 6, 1-9. doi: 10.4236/oalib.1105771.