Author(s)

Mohamed Moustapha^1*, Molka Chadhli-Chaieb², Touhami Mahjoub³, Larbi Chaieb²

¹Internal Medicine Department, Hopital Cheikh Zayed, Nouakchott, Mauritania.
²Endocrinology Department, CHU Farhat Hachad, Sousse, Tunisia.
³Reserche Unit of Biology, Genetic of Cancer, Haematological and Autoimmune Disease, Monastir Faculty of Pharmacy, Monastir, Tunisia.

Background: The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor; plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma PAI-1 levels, with 4G carriers having high PAI-1 level than 5G, theses pose the question about relation T2 patients and those polymorphisms. The aim of this study was to determine the relationship between the polymorphisms −675 4G/5G and −844 G/A of PAI-1 gene and type 2 diabetes mellitus. Methods: A case control study of 491 diabetic and 400 healthy controls. Genotyping of the polymorphism −675 4G/5G was done by PCR-ASA (polymerase chain reaction, allele specific amplification), and the polymorphism −844 G/A was done with PCR-RFLP (restriction fragment length polymorphism), the allelic frequency is calculated with hardy-Weinberg law, the statistic analysis was done by SPSS version 10. Results: Higher frequencies of The genotypes 4G/4G (p = 0.01) and 4G/5G of polymorphism −675 4G/5G were seen in diabetic (p = 0.05) and higher frequencies of 5G/5G was seen in controls (p < 0.001). Higher frequencies of the genotype A/A (p < 0.01) and G/A (p = NS) of polymorphism −844 G/A was seen in diabetics and G/G was seen in controls (p = 0.01). Conclusion: Our study found association between 4G allele of −675 4G/5G and A allele of −844 G/A of PAI-1 gene and having type 2 diabetes mellitus in Tunisian population.

Plasminogéne Activator Inhbitor-1 (PAI-1), −675 4G/5G Polymorphism, −844 G/A Polymorphism, Type 2 Diabetes Mellitus

Moustapha, M. , Chadhli-Chaieb, M. , Mahjoub, T. and Chaieb, L. (2019) −675 4G/5G and −844 G/A of Plasminogne Activator Inhibitor-1 (Pai-1) Gene Polymorphisms and Type 2 Diabetes Mellitus in Tunisia: Case-Control Study. Open Journal of Endocrine and Metabolic Diseases, 9, 75-83. doi: 10.4236/ojemd.2019.97008.