Author(s)

Olugbenga Adeola Odukanmi^*, Queenneth Blessing Olusegun, Samuel Babafemi Olaleye

Laboratory for Gastrointestinal Secretion and Inflammation Research, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.

Background: Different studies have unveiled the traditional usefulness and clinical potentials of Buchholzia coriacea, a medicinal plant known for its effectiveness in lowering blood glucose. Its role in intestinal glucose uptake was investigated. Materials and methods: Thirty male Wistar rats, weighing between 100 - 120 g were used and randomly assigned into three groups of 5 rats each per experiment. Group 1: control (not treated), groups 2 and 3, were treated with 100 mg/kg (BC100) and 200 mg/kg (BC200) of Buchholzia coriacea orally respectively for 2 weeks. Fasting blood glucose, luminal and in vitro glucose levels of rats were determined by glucose oxidase method using glucometer stripes plus glucose monitoring system (Fine test glucometer^(R) ). Luminal electrolytes in the in vitro study were determined by Atomic Absorption Spectrophotometry method. Data were expressed as Mean ± SEM and statistical analysis was by one way ANOVA, and p-values < 0.05 were considered significant. Results: There was no significant change in the fasting blood glucose level (mg/dl) of rats in BC100 (78.00 ± 2.16) and BC200 (76.0 ± 3.57) compared with control (79.50 ± 1.70). There was significant increase in glucose uptake (mg/dl/g tissue) in the in vivo experiments in both the ileum and jejunum of BC100 (23.08 ± 0.18; 19.68 ± 0.72) and BC200 (14.50 ± 1.02; 20.55 ± 0.45) compared with control (30.40 ± 1.01; 35.53 ± 1.45), respectively. The glucose uptake at the mucosa end of distal jejunum in the BC100 (292 ± 3.33) and BC200 (209.30 ± 2.67) decreased significantly compared with control (90.00 ± 1.50). There was a significant decrease in K⁺ concentration and increase in Na⁺ concentration at the mucosa end in the BC100 and BC200 compared with control. Conclusion: The study suggests glucose stowing in the intestinal cells in the in vivo study and inhibition of glucose transport from the in vitro study. The roles of alpha-amylase on the activity of this extract are suggested for future studies.

Buchholzia coriacea, Intestine, Glucose Uptake, Electrolytes, Rats

Odukanmi, O. , Olusegun, Q. and Olaleye, S. (2019) Intestinal Handling of Glucose in Buccholzia coriacea Treated Male Wistar Rats. Journal of Biosciences and Medicines, 7, 87-98. doi: 10.4236/jbm.2019.77007.