Crude of Plant extracts from materials is necessary screening to determine new biological activities, toxicity and active compounds in bioassay tests. Crude of Populus euphratica L. left is tested to phytochemical screening, constituents, fractionation and MIC technique as anti-fungal. The fractions are subjected to bio-compound analysis using Liquid chromatography-mass spectrometry LC-MS. Phytochemical screening of crude extract shows presence of Glycosides compared with other compounds. While the fraction of ethanolic extract recorded inhibition impact reached (390, mg/L/MIC) agnist type of fungi. Bioassay toxicity using shrimp lethally test (LC₅₀) was carried out with the result showing 4.1 mg/L is low toxic and can be used safely. Alpha Glycoside (4, 6 Benzylidene-1O-Methyl-2O-(2346 Tetra-O-Acetyl-Betad- Glucosyl), is a new compound and antifungal action.