RNA-Seq and Bulked Segregant Analysis of Genes Related to High Growth in Ginkgo biloba Half-Sibling Families - American Journal of Plant Sciences

AJPS > Vol.10 No.1, January 2019

American Journal of Plant Sciences

Volume 10, Issue 1 (January 2019)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

RNA-Seq and Bulked Segregant Analysis of Genes Related to High Growth in Ginkgo biloba Half-Sibling Families ()

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DOI: 10.4236/ajps.2019.101008 1,219 Downloads 2,442 Views Citations

Author(s)

Limin Sun¹, Haixia Tang², Xiaoyan Men¹, Qian Zhang³, Xia Sun^4*, Shiyan Xing^1*

Affiliation(s)

¹Key Laboratory of Tree Germplasm Resources Research, College of Forestry, Shandong Agricultural University, Tai’an, China.
²Shandong Institute of Pomology, Tai’an, China.
³Taishan Academy of Forestry Sciences Tai’an City, Tai’an, China.
⁴College of Horticulture Science and Engineering, Shandong Agriculture University, Tai’an, China.

ABSTRACT

The lifetime of G. biloba is very long, and its growth is relatively slow. However, little is known about growth-related genes in this species. We combined mRNA sequencing (RNA-Seq) with bulked segregant analysis (BSA) to fine map significant agronomic trait genes by developing polymorphism molecular markers at the transcriptome level. In this study, transcriptome sequencing of high growth (GD) and low growth (BD) samples of G. biloba half-sib families was performed. After assembling the clean reads, 601 differential expression genes were detected and 513 of them were assigned functional annotations. Single nucleotide polymorphism (SNP) analysis identified SNPs associated with 119 genes in the GD and BD groups; 58 of these genes were annotated. Two Homeobox-leucine zipper protein genes were up-regulated in the GD group compared with the BD group; therefore, these are very likely related to high growth of G. biloba. This study provides molecular level data that could be used for seed selection of high growth G. biloba half-sib families for future breeding programs.

KEYWORDS

High Growth, Ginkgo biloba Half-Sibling Families, RNA-Seq and Bulked Segregant Analysis, the Transcriptome Sequencing, Differentially Expressed Genes

Share and Cite:

Sun, L. , Tang, H. , Men, X. , Zhang, Q. , Sun, X. and Xing, S. (2019) RNA-Seq and Bulked Segregant Analysis of Genes Related to High Growth in Ginkgo biloba Half-Sibling Families. American Journal of Plant Sciences, 10, 79-100. doi: 10.4236/ajps.2019.101008.

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