Author(s)

Tatsuya Fukuda, In-Ja Song, Hokuto Nakayama, Takuro Ito, Akira Kanno, Hiroshi Hayakawa, Yukio Minamiya, Jun Yoko Yama

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To describe the phylogeographic structures of Asparagus schoberioides Kunth (Asparagaceae) in Japan, we investigated its nucleotide sequence variations with respect to its geographic distribution pattern. Sequencing of the internal transcribed spacer (ITS) 1 region in 29 samples of A. schoberioides revealed 20 polymorphic nucleotide sites. As a result, the 29 samples of A. schoberioides fell into 15 distinct haplotypes and phylogenetic analyses revealed these haplotypes fell into two major clades, Clade 1 and Clade 2. The haplotypes of Clade 1 were distributed chiefly along the Pacific Ocean side of Japan, while those of Clade 2 occurred mainly along the Japan Sea side. This result suggests that A. schoberioides has migrated via two routes in Japan.

Asparagus schoberioides, Internal Transcribed Spacer (ITS), Phylogeny, Phylogeography

T. Fukuda, I. Song, H. Nakayama, T. Ito, A. Kanno, H. Hayakawa, Y. Minamiya and J. Yama, "Phylogeography of Asparagus schoberioides Kunth (Asparagaceae) in Japan," American Journal of Plant Sciences, Vol. 2 No. 6, 2011, pp. 781-789. doi: 10.4236/ajps.2011.26093.