Author(s)

Keiichi Uchida, Kohei Otake, Mikihiro Inoue, Yuhki Koike, Kohei Matsushita, Koji Tanaka, Yasuhiro Inoue, Yasuhiko Mohri, Masato Kusunoki

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Purpose: The purpose of this study is to determine the presence of bacteriostatic effects of hyaluronan-based bioresorbable membrane (HA/CMC) on selected major bacterial strains in digestive organs. Methods: We firstly evaluated the growth inhibition effect of HA/CMC for E. coli and S. aureus by determining the optical density (OD)₆₅₀ in the incubation medium. At second, to determine the viable counts of bacteria, total adenosine triphosphate (ATP) were measured with five groups; several concentrations of HA/CMC and control. Results: OD curve gradually elevated and reached to plateau at 4 hours in E. coli. and 6 hours in S. aureus. After reaching plateau, the growth inhibition of both strains was statistically significantly correlated to the concentrations of HA/CMC. The ATP productions had statistically significant differences at 6 hours after incubation and inhibited in dose-dependence of a well-dissolved HA/CMC. Conclusion: HA/CMC may have dose-dependently bacteriostatic effects on S. aureus and E. coli.

HA/CMC, Bacteriostatic, Hyaluronan, Adhesion, Surgery

K. Uchida, K. Otake, M. Inoue, Y. Koike, K. Matsushita, K. Tanaka, Y. Inoue, Y. Mohri and M. Kusunoki, "Bacteriostatic Effects of Hyaluronan-Based Bioresorbable Membrane," Surgical Science, Vol. 2 No. 9, 2011, pp. 431-436. doi: 10.4236/ss.2011.29094.