Screening of Single Nucleotide Polymorphism in CD95 (APO-1/FAS) Promoter Region (G-1377A) in Children with Acute Leukemia ()
Affiliation(s)
1Faculty of Medicine, Medical Biology, Nigde Omer Halisdemir University, Nigde, Turkey.
2Vocational School of Health Service, Okan University, Istanbul, Turkey.
3LOSANTE Children’s and Adult Hospital, Ankara, Turkey.
4TOBB-ETU Hospital, Ankara, Turkey.
ABSTRACT
CD95 is a cell surface receptor involved in apoptotic signal transmission. Deregulation of this pathway results in down regulation of apoptosis and subsequent persistence of a malignant clone. A single nucleotide polymorphism (SNP) resulting in guanine-to-adenine (G > A) transition in the CD95 promoter region (position ﹣1377) is thought to reduce stimulatory protein 1 (SP1) transcription factor binding and decrease CD95 expression. The purpose of this study was to examine a genetic polymorphism in the core promoter of CD95 is associated with the frequency. G-1377A in the CD95 promoter region was genotyped by polymerase chain reaction and restriction endonuclease analysis and sequenced of the CD95 gene (Beckman Coulter, USA). Among 146 patients, CD95 G-1377A (rs2234767) SNP carriers frequencies has been identified as 25% GA (n:37) and AA 4% (n:6), respectively. This polymorphism of the distribution of the CD95 gene in acute leukemic children will be a guide for future studies.
Share and Cite:
Bali, D. , Ozkan, D. , Kurekçi, A. and Akar, N. (2018) Screening of Single Nucleotide Polymorphism in CD95 (APO-1/FAS) Promoter Region (G-1377A) in Children with Acute Leukemia.
Open Access Library Journal,
5, 1-7. doi:
10.4236/oalib.1104385.
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