Detection of Antibody in Dogs with Blastomycosis Using Blastomyces dermatitidis Yeast Phase Lysate Antigens ()
ABSTRACT
The objective of our study was to compare two B. dermatitidis yeast phase lysate antigens [ERC-2, dog Wisconsin; 85, soil Georgia, ATCC 56,920] for detecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. The mean absorbance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. The mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418; Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452; 85 had an absorbance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150; 85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. This study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen.
Share and Cite:
Kennell, B. , Scalarone, G. , Hoyos-Carvajal, L. and RoyChowdhury, M. (2017) Detection of Antibody in Dogs with Blastomycosis Using
Blastomyces dermatitidis Yeast Phase Lysate Antigens.
Open Journal of Veterinary Medicine,
7, 111-119. doi:
10.4236/ojvm.2017.79011.