Author(s)

Silvia Reina^1,2, Cecilia Pisoni³, Roberto Arana³, Sabrina Ganzinelli^1,4, Enri Borda^1,4

¹National Research Council of Argentina (CONICET), Buenos Aires, Argentina.
²University of Catholic Mision, Misiones, Argentina, Department of Internal Medicine, CEMIC, Buenos Aires Argentina.
³Section of Rheumatology and Immunology, Department of Internal Medicine, CEMIC, Buenos Aires Argentina.
⁴Pharmacology Unit, School of Dentistry, University of Buenos Aires.

Aims: This paper investigates the presence of M₃ muscarinic acetylcholine receptor autoantibody present in the serum of patients with primary SjÖgren syndrome (pSS). Main methods: We detected the levels of M₃mAChR peptide IgG, PGE₂, IL-1β in serum of SS patients using the enzyme-linked immune sorbent assay (ELISA). To measure the quantity of nitrite/nitrate, we used Griess reagent system. Key findings: Titres of M₃mAChR antibody in sera from SS patients are significantly enhanced compared to healthy subjects (control). The enhancement of these autoantibodies is accompanied by the increase of the levels of PGE₂, IL-1β and nitrite/nitrate in serum. Under in vitro conditions, the synthetic human M₃ peptide impaires the increment of M₃mAChR antibody but not that of nati-Ro/SSA antibody. In positive anti-Ro/SSA antibody patients, the increment of M₃mAChR peptide IgG and the measured pro-inflammatory substances is related. Significance: On this basis, anti M₃mAChR peptide IgG can be said to act as a modulator of the immune system and to play a role in the host-chronic increment of proinflammatory substances in SS patients with positive Ro/SSA antibody. This association between the antibody and the pathogenesis of SS disease may result in useful predicting SS.

Anti-Ro/SSA Antibody, Anti M3mAChR Peptide IgG, IL-1β, PGE2, Nitric Oxide

Reina, S. , Pisoni, C. , Arana, R. , Ganzinelli, S. and Borda, E. (2017) Role of M₃ Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome. Pharmacology & Pharmacy, 8, 242-252. doi: 10.4236/pp.2017.87017.