Author(s)

Itaru Takeda^1,2, Hiroya Itoh³, Makoto Matsui³, Takashi Shibata³, Masayuki Machida^1,4, Sachiyo Aburatani^2,5*

¹Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
²Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
³Astellas Pharma Inc., Tsukuba Biotechnology Research Center, Ibaraki, Japan.
⁴Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan.
⁵Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), Tokyo, Japan.

Secondary metabolites are important for various industrial applications. The production of secondary metabolites is often improved by the activation of substrate supply pathways for biosynthesis. However, many important pathways have remained unclear. In this study, we explored possible pathways related to substrate supply for the biosynthesis of the antifungal agent FR901469 which is a nonribosomal peptide and a fungal secondary metabolite. To clarify the unknown activated pathways, we utilized the Comprehensive Pathway Model (CPM) which was developed in our previous study. We verified that the overexpression of the hypothetical beta-alanine-aminotransferase (BAL-AT), which was included in the explored pathways, improved the FR901469 productivity. The genes encoding the BAL metabolic enzymes are considered to be important for improving the FR901469 productivity.

Bioinformatics, Metabolic Pathway Model, Fungi, Secondary Metabolites

Takeda, I. , Itoh, H. , Matsui, M. , Shibata, T. , Machida, M. and Aburatani, S. (2017) Exploration of Activated Pathways for Improving Antifungal Agent FR901469 Productivity in Fungal Species No.11243 Using Comprehensive Pathway Model. Journal of Biosciences and Medicines, 5, 16-31. doi: 10.4236/jbm.2017.57003.