Assessment of Genetic Diversity in Contrasting Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers - American Journal of Plant Sciences

AJPS > Vol.2 No.3, September 2011

American Journal of Plant Sciences

Volume 2, Issue 3 (September 2011)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Assessment of Genetic Diversity in Contrasting Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers ()

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DOI: 10.4236/ajps.2011.23048 8,927 Downloads 16,022 Views Citations

Author(s)

Maria Lucília M. da Costa, Lidiane L. Barbosa Amorim, Alberto V. C. Onofre, Luiz J. O. Tavares de Melo, Maria Betânia M. de Oliveira, Reginaldo de Carvalho, Ana M. Benko-Iseppon

Affiliation(s)

ABSTRACT

Sugarcane is an important tropical crop, responsible for two thirds of the world sugar production, gaining actually importance as a source of biofuel. Drought tolerance is a very important feature considering the actual climate change scenario throughout the world. This study aimed to determine the genetic diversity between sugarcane varieties with contrasting features under drought. For this purpose, twelve ISSR primers were used to characterize nine sugarcane varieties under cultivation in different countries including selected drought resistant material from Northeast Brazil and two varieties from India as contrasting genotypes. 317 scorable bands were generated, among which 301 comprised polymorphic markers, with an average of 25 polymorphic bands per primer. In the generated dendrogram the accessions were placed in clusters, where cluster A included two varieties from India (Co331 and Co419), and B comprised plants eight Brazilian accessions and a ‘Barbado’ variety. Within this clade, drought tolerant and susceptible varieties were clearly separated. The present evaluation revealed important contrasting parental candidates regarding their drought response, very promising for future mapping approaches aiming the identification of quantitative trait loci (QTLs) associated to drought in sugarcane. The selected primers were used for the first time in sugarcane, representing valuable tools for future evaluations, with emphasis to diversity characterization and genetic mapping.

KEYWORDS

Saccharum, Genetic Variability, ISSR Marker, Drought Tolerance

Share and Cite:

Costa, M. , Amorim, L. , Onofre, A. , Melo, L. , Oliveira, M. , Carvalho, R. and Benko-Iseppon, A. (2011) Assessment of Genetic Diversity in Contrasting Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers. American Journal of Plant Sciences, 2, 425-432. doi: 10.4236/ajps.2011.23048.

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