ABSTRACT
Doryopteris triphylla (Pteridaceae-Cheilanthoideae) grows in xeric
habitats in Brazil, Paraguay, Uruguay and Argentina. The aim of this study was to characterize D. triphylla anatomically, histochemically and cytogenetically. For
anatomical characterization, rhizomes, roots, petioles and leaves were made and
then stained using Safranine-Astra Blue for further observations. Leaf blades
were also cleared. For histochemical analysis, leaf cross sections were stained
with different reagents to identify glandular trichomes compounds. For cytogenetic
characterization, a karyogram was performed using laboratory cultivated roots.
Results show a dictyostelic rhizome covered with scales with apical secreting
gland; diarch roots; petiole cross-sections show thick cuticle, uniseriate
epidermis, parenchymatic cortex cells with thick walls and a vascular bundle
with two xylem groups; and hypostomatic fronds with glandular trichomes.
Histochemical studies of secretion products of the glandular trichomes were
positive for polysaccharides, pectins, lipids, acid lipids, dihydroxyphenols,
phenols and flavonoids. Cytogenetically, D.
triphylla is described as a diploid species (2n = 60),
with chromosomes gradually decreasing in size. The apical glands in scales of
rhizomes, the presence of two xylem groups in the vascular bundle in the
petiole and the glandular trichomes on the abaxial surface are new
contributions to the species. The type of chemical products secreted by glandular
leaf trichomes and karyotype estimation is shown for the first time in this species.
Share and Cite:
Neira, D. , Andrada, A. , de los Ángeles Páez, V. , Rodriguez, A. , Ríos, N. , Martínez, O. and Hernández, M. (2017) Anatomical, Histochemical and Cytogenetic Features of
Doryopteris triphylla (Pteridaceae).
American Journal of Plant Sciences,
8, 907-920. doi:
10.4236/ajps.2017.84061.