Author(s)

Masato Hotta^*, Daisuke Tamura, Hirotomo Kotake, Shusuke Kusakabe, Taiyu Gen, Kazuki Oike

Division of Oral Functional Science and Rehabilitation, Department of Operative Dentistry, Asahi University School of Dentistry, Mizuho, Gifu, Japan.

The purpose of this study was to measure the amount of adsorption of various salivary proteins to a resin composite having various amounts of surface pre-reacted glass-ionomer (S-PRG) fillers, and to make a comparative study of the adherence of S. mutans to the resin composite covered by various salivary proteins. We experimentally produced resin composites (S-PRG resin) having the basic composition of Bis-GMA/TEGDMA and various amount of the S-PRG fillers ranging between 0 - 60 wt%. Each S-PRG resin block was soaked in 5 kinds of components found in salivary fluid (Mucin 1, Lactoferrin, IgA, Cystatin C, and Lysozyme), and the amount of adsorption was measured by use of a spectrophotometer. The amount of the adsorption of salivary Mucin 1 was higher than that of any other salivary component tested regardless of the percentage of the S-PRG filler. In the case of salivary Lysoxyme used for coating, the amount of its adsorption increased with an increase in the percentage of the S-PRG filler. In addition, resin blocks coated with various salivary proteins were incubated at 37℃ for 2 hours with radio-labeled S. mutans for a quantitative adherence test. Labeled bacteria that adhered to the resin blocks were collected by using an automatic sample combustion system and a liquid scintillation counter. The absorbed salivary components, especially Mucin 1 and Lysozyme, inhibited the adhesion of S. mutans to the S-PRG resin; however, these changes were generally directional rather than statistically significant.

S-PRG Filler, Salivary Protein, Oral Bacteria, Resin Composite

Hotta, M. , Tamura, D. , Kotake, H. , Kusakabe, S. , Gen, T. and Oike, K. (2017) Adherence of Streptococcus mutans and Adsorption of Salivary Protein to Resin Composites Containing S-PRG Fillers. Open Journal of Stomatology, 7, 158-168. doi: 10.4236/ojst.2017.73011.