Author(s)

Lei Hou¹, Xiuying Zhang², Yang Li³, Shuai Chen³, Hongyi Qu⁴, Jiazhi Yu³, Lianhai Zhang⁵, Ziyi Fan^1*

¹Department of Thyroid and Breast Surgery, General Hospital of Jinan Military Command, Jinan, China.
²Shandong Prosthetics Orthotics Rehabilitation Center, Jinan, China.
³Qilu Hospital of Shandong University, Jinan, China.
⁴Qianfo Mt. Hospital of Shandong University, Jinan, China.
⁵Liao City Municipal Hospital, Liaocheng, China.

Quantitative real-time PCR (qPCR) was applied to rapid screening of positive plasmid clones. Insert-specific primer pairs were used in qPCR colony screening, and false positive colonies could easily be distinguished from true positive ones by comparing their Ct values. In addition, qPCR is particularly suitable when amplicon is small (<150 bp). This method is sensitive, simple and fast, obviates the need for gel electrophoresis, and is a cost-effective alternative to the traditional PCR approach.

qPCR, Colony PCR, Plasmid Screening

Hou, L. , Zhang, X. , Li, Y. , Chen, S. , Qu, H. , Yu, J. , Zhang, L. and Fan, Z. (2016) Rapid Screening of Recombinant Plasmids by Direct Colony Quantitative Real-Time PCR. Advances in Bioscience and Biotechnology, 7, 428-433. doi: 10.4236/abb.2016.710041.