Author(s)

Ling-Ling Zhao, Jun-Le Qu, Dan-Ni Chen, Han-Ben Niu

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education, Institute of Optoelectronics, Shenzhen University, Shenzhen, China..

In this paper, we present our investigation on the morphological and autofluorescence char-acteristics of the cones and rods using two- photon excitation with a femtosecond Ti: sap-phire laser. The results show that the micro-structures of the photoreceptor layers can be visualized at submicron level without any stain- ing or slicing. The morphology and spatial dis-tribution of the cones and rods can be resolved by autofluorescence imaging. The autofluores-cence in the photoreceptor outer segments is much stronger than other layers, but suscepti-ble to light-induced damage.

Two-Photon Excitation; Photoreceptor; Retina; Autofluorescence

Zhao, L. , Qu, J. , Chen, D. and Niu, H. (2009) Layered-resolved autofluorescence imaging of photo-receptors using two-photon excitation. Journal of Biomedical Science and Engineering, 2, 363-365. doi: 10.4236/jbise.2009.25052.