Author(s)

Damien S. Tshibangu¹, Selvaraj Divakar², Muthiah Ramanathan², Govindarajan Syamala², Koto-te-Nyiwa Ngbolua³, Jean Chrysostome V. Mudogo¹, Dinangayi D. Tshilanda¹, Nicole M. Misengabu⁴, Pius T. Mpiana^1*

¹Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
²Department of Pharmacology, PSG College of Pharmacy, Coimbatore, India.
³Molecular Biology Unit, Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
⁴Department of Biology, Institut de Recherche en Sciences de la Santé, Kinshasa, Democratic Republic of the Congo.

In this study, we evaluated the anti-cancer property of five bark extracts and the isolates from chloroform and ethyl acetate of Annickia chlorantha by the tetrazolium salt method (MTT method). The anti-cancer activity was performed on human prostate cancer cell lines PC-3 and hormone-dependent breast cancer cell lines MCF-7. Results indicated that the two isolates displayed interesting cytotoxicity towards MCF-7 cell lines with CC₅₀ of 3.84 CC₅₀/mL and 4.87 CC₅₀/mL for chloroform and ethyl acetate respectively; while the total bark extracts showed CC₅₀ of 24.33 CC₅₀/mL, 36.49 CC₅₀/ mL and 73.52 CC₅₀/mL for chloroform, ethyl acetate and methanol extracts respectively. By the other hand on PC-3, the CC₅₀ of the isolates were higher than the one on MCF-7, more than 10 CC₅₀/mL for both chloroform and ethyl acetate isolates and 49.14 CC₅₀/mL, 77.33 CC₅₀/mL, 89.38 CC₅₀/mL and 92.37 CC₅₀/mL, respectively for chloroform, ethyl acetate and methanol soluble extracts. From this study, we identified that the two isolates had anti-cancer properties against MCF-7 cell lines.

Annickia chlorantha, Cancer, MCF-7 Cell Lines, PC-3 Cell Lines, Anti-Proliferative Compounds

Tshibangu, D. , Divakar, S. , Ramanathan, M. , Syamala, G. , Ngbolua, K. , Mudogo, J. , Tshilanda, D. , Misengabu, N. and Mpiana, P. (2016) In Vitro Anticancer Assessment of Annickia chlorantha (Oliv.) Setten & Maas Stem (Annonaceae) Bark from Democratic Republic of Congo. Journal of Biosciences and Medicines, 4, 23-29. doi: 10.4236/jbm.2016.44004.