Advances in Bioscience and Biotechnology

Volume 6, Issue 9 (September 2015)

ISSN Print: 2156-8456   ISSN Online: 2156-8502

Google-based Impact Factor: 1.09  Citations  h5-index & Ranking

Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)*

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DOI: 10.4236/abb.2015.69065    4,281 Downloads   4,731 Views  

ABSTRACT

The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.

Cite this paper

Xin, L. , Hou, L. , Zhou, Y. , Wang, H. and Yu, H. (2015) Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)*. Advances in Bioscience and Biotechnology, 6, 624-628. doi: 10.4236/abb.2015.69065.

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