Anti-Inflammatory Pre-Treatment to Reduce Mobilization-Induced Liver Inflammation in Mice: Novel Model to Study Liver Injury ()
Author(s)
T. Hendrikx1,2,
C. Piersma3,
D. K. Dhar4,5,
L. Mpabanzi2,3,
J. J. W. Schreurs1,2,
M. Malagó4,
R. Shiri-Sverdlov1,2,
S. W. M. Olde Damink2,3,4*
Affiliation(s)
1Department of Molecular Genetics, Maastricht University, Maastricht, The Netherlands.
2Nutrition and Toxicology Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.
3Department of Surgery, Maastricht University Medical Centre, Maastricht, The Netherlands.
4Department of HPB Surgery and Liver Transplantation, Royal Free Hospital, University College London, London, UK.
5Comparative Medicine Department, Organ Transplantation Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
ABSTRACT
Background: Liver surgery requires mobilization of the liver resulting in
injury. Mobilization is the predominant cause of hepatocyte damage during liver
surgery and jeopardizes post-operative liver function. Previously,
mobilization-induced liver injury was found to be associated with inflammation.
So far, anti-inflammatory drugs to potentially prevent liver inflammation
following liver mobilization were not tested. In this study, we aimed to establish an in vivo mouse
model of mobilization-induced liver injury and to evaluate the effect of
anti-inflammatory pre-treatment before liver mobilization on liver
inflammation. Methods: To develop a mouse model for mobilization-induced liver
injury, C57BL/6 mice (n = 8) underwent surgery during which the liver was
mobilized by gentle manipulation of the lobes with cotton-wool applicators for
15 minutes. In two control groups, the liver was left alone or was subjected to
laparotomy only. An additional group was added that received anti-TNF treatment
(Infliximab) 2 days prior to surgery. Liver samples were obtained 2 hours after
mobilization and liver inflammation was analyzed by histology and inflammatory
gene expression. Results: Gentle liver mobilization resulted in acute liver
inflammation as indicated by increased recruitment of inflammatory cells and
elevated inflammatory gene expression compared to controls. Infliximab
pre-treatment had no effect on the inflammatory response in the liver. Conclusion:
Our current model provides an excellent opportunity to study the effects of
pre-treatment with anti-inflammatory drugs on mobilization-induced liver
inflammation. Further studies are needed to investigate whether anti-inflammatory
mediators can be used to prevent liver inflammation.
Share and Cite:
Hendrikx, T. , Piersma, C. , Dhar, D. , Mpabanzi, L. , Schreurs, J. , Malagó, M. , Shiri-Sverdlov, R. and Damink, S. (2015) Anti-Inflammatory Pre-Treatment to Reduce Mobilization-Induced Liver Inflammation in Mice: Novel Model to Study Liver Injury.
Modern Research in Inflammation,
4, 1-7. doi:
10.4236/mri.2015.41001.
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