Cytokinin, Carbon Source, and Acclimatization Requirements for in Vitro Propagation of Scutellaria barbata D. Don and Scutellaria racemosa Pers. - American Journal of Plant Sciences

AJPS > Vol.5 No.24, November 2014

Volume 5, Issue 24 (November 2014)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Cytokinin, Carbon Source, and Acclimatization Requirements for in Vitro Propagation of Scutellaria barbata D. Don and Scutellaria racemosa Pers. ()

HTML XML

Download as PDF (Size: 3081KB) PP. 3662-3672

DOI: 10.4236/ajps.2014.524382 3,486 Downloads 4,580 Views Citations

Author(s)

Terri Ann Brearley, Brajesh Nanda Vaidya, Nirmal Joshee^*

Affiliation(s)

Agricultural Research Station, Fort Valley State University, Fort Valley, GA, USA.

ABSTRACT

Micropropagation protocols to minimize hyperhydricity were optimized for medicinal Scutellaria barbata and Scutellaria racemosa. Six cytokinins and eight different carbon sources at two different incubation periods of 14 and 21 days were studied for adventitious shoot bud induction using nodal explants. In S. barbata, 5 μM meta-Topolin and 0.1 μM NAA supplemented shoot induction medium produced four shoots each after 14 and 21 day incubation. Observation of S. racemosa nodal explants recorded four and five shoots after 14 and 21 day incubation. In both species, control explants (no plant growth regulators in the medium) consistently resulted in the bud break with two shoots in both 14 and 21 day incubation. The effect of carbon source on shoot regeneration was studied by supplementing eight different sugars at 0.1 M concentration to the optimized shoot induction medium (5 μM meta-Topolin and 0.1 μM NAA). S. barbata nodal explants cultured on shoot induction medium supplemented with fructose and glucose for 14 days produced 10 and nine adventitious shoots respectively; and after 21 day incubation adventitious shoot count reached 19 in glucose supplemented medium. S. racemosa explants in the same experiment produced five shoots in maltose and four shoots in sorbitol supplemented medium after 14 day incubation; whereas after 21 day incubation, sucrose and maltose produced five shoots; fructose, glucose, and sorbitol produced four shoots. Regenerated plants were successfully acclimatized and Scanning Electron Microscopy of the leaf surface revealed differences in stomatal behavior and cuticle deposition between in vitro and acclimatized plants. The antioxidant assay conducted on both Scutellaria species showed considerable total polyphenol content, TEAC activity and flavonoid content in fresh and dried leaf samples attributing to their medicinal potential.

KEYWORDS

Antioxidant, 6-Benzylaminopurine, Flavonoid, meta-Topolin, Polyphenol, RIM, SIM

Share and Cite:

Brearley, T. , Vaidya, B. and Joshee, N. (2014) Cytokinin, Carbon Source, and Acclimatization Requirements for in Vitro Propagation of Scutellaria barbata D. Don and Scutellaria racemosa Pers.. American Journal of Plant Sciences, 5, 3662-3672. doi: 10.4236/ajps.2014.524382.

Cited by

[1]	Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers
	Siemińska - Genes, 2022

[2]	Micropropagation of Rare Scutellaria havanensis Jacq. and Preliminary Studies on Antioxidant Capacity and Anti-Cancer Potential
	Molecules, 2021

[3]	Role of Meta-topolin on in Vitro Shoot Regeneration: An Insight
	2021

[4]	Meta-topolina: uma alternativa para a prevenção do estresse oxidativo na micropropagação da cana-de-açúcar
	Hoehnea, 2019

[5]	Improving micropropagation of Mentha × piperita L. using a liquid culture system
	2019

[6]	Meta-topolin: an alternative for the prevention of oxidative stress in sugarcane micropropagation
	2019

[7]	Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of Anoectochilus elatus Lindl., an endangered …
	Plant Cell, Tissue and Organ Culture (PCTOC), 2018

[8]	Anticancer Plants and Their Conservation Strategies: An Update
	Anticancer plants: Properties and Application, 2018

[9]	An Untapped Resource in the Spotlight of Medicinal Biotechnology: The Genus Scutellaria
	Current Pharmaceutical Biotechnology, 2018

[10]	Thin Cell Layer (TCL) Culture System for Herbal Biomass Production and Genetic Transformation of Bacopa monnieri L. Wettst.
	2016

[11]	降低黄芩再生苗玻璃化率及其生根的研究
	2016

[12]	Agrobacterium-mediated transformation of thin cell layer explants of Scutellaria ocmulgee small: a rare plant with anti-tumor properties
	Plant Cell, Tissue and Organ Culture (PCTOC), 2016

[13]	Micropropagation and DNA Barcoding Studies in the Genus Scutellaria
	2016

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies