Open Journal of Animal Sciences

Volume 4, Issue 5 (October 2014)

ISSN Print: 2161-7597   ISSN Online: 2161-7627

Google-based Impact Factor: 1.35  Citations  

Effect of Indoor Concentrate Feeding vs. Outdoor Grazing on the Expression of Genes Involved in Muscle Growth and Nutrient Content in Japanese Black Steer Muscle

HTML  XML Download Download as PDF (Size: 2694KB)  PP. 297-304  
DOI: 10.4236/ojas.2014.45038    3,579 Downloads   4,628 Views  Citations

ABSTRACT

The objective of the present study was to investigate the effect of outdoor grazing on the expression of genes involved in muscle growth and the nutrient contents of skeletal muscle in steers. Ten Japanese Black steers were divided into two groups: grazing (GR) and concentrate (CT) groups. Crude protein, extractable lipid, moisture, fatty acid, cooking loss and Warner Bratzler shear force in muscle tissue were analyzed. The gene expression of myosin heavy chain (MyHC) isoform (2a, 2x and slow), myostatin, follistatin, peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα), heat shock protein (HSP) 27 and HSP40 in skeletal muscles was evaluated at the end of fattening. Decreases in MyHC-2a and MyHC-2x (fast-twitch fiber type) expression in the longissimus lumborum (LL) muscle were detected in the GR group compared with the CT group; in contrast, an increase in MyHC-slow (slow-twitch fiber type) expression was shown in the GR group. These results suggest that grazing initiated muscle fiber type conversion to slow-twitch from fast-twitch. A decrease in extractable lipid content was observed in the GR group in the LL and semitendinosus (ST) muscles. Crude protein content in the LL muscle in the GR group was higher than in the CT group. MyHC expression in LL muscle in the GR group was greater than in the CT group. A decrease in myostatin and PPARγ2 gene expression was detected in the GR group compared with the CT group in both muscles. Expression of C/EBPα in LL muscle in the GR group was lower than in the CT group. These results suggest that grazing steers at the end of fattening may lead to an increase in protein content and a decrease in fat accumulation in LL and/or ST muscles by regulation of myostatin, MyHC, PPARγ2 and C/EBPα gene expression.

Share and Cite:

Shibata, M. , Matsumoto, K. , Hikino, Y. and Yamamoto, N. (2014) Effect of Indoor Concentrate Feeding vs. Outdoor Grazing on the Expression of Genes Involved in Muscle Growth and Nutrient Content in Japanese Black Steer Muscle. Open Journal of Animal Sciences, 4, 297-304. doi: 10.4236/ojas.2014.45038.

Cited by

[1] Expression of PLCZ1 mRNA in spermatozoa of Criollo and European bulls in a low-input system
Roacho, FA Rodriguez-Almeida… - Animal …, 2023
[2] 筋肉内遺伝子発現を活用した多様な肉用牛肥育技術の開発に関する研究
2020
[3] Evaluación de estrategias de alimentación para la producción de carne de alta calidad con animales provenientes de lechería utilizando tres tipos …
2019
[4] Comprehensive evaluation of growth performance and meat characteristics of a fattening system combining grazing with feeding rice whole‐crop silage in Japanese …
2019
[5] Evaluación de estrategias de alimentación para la producción de carne de alta calidad con animales provenientes de lechería utilizando tres tipos diferentes de …
2019
[6] Muscle fibre type and beef quality
2017
[7] Relationship between animal diet with color and tenderness beef cattle.
2017
[8] Muscle fibre types and beef quality
Ensuring safety and quality in the production of beef Volume 2, 2017
[9] Relación de la dieta con el color y la terneza de la carne vacuna
2017
[10] Differences in circulating microRNAs between grazing and grain-fed wagyu cattle are associated with altered expression of intramuscular microRNA, the …
2016
[11] Impacts of environment on gene expression and epigenetic modification in grazing animals
Journal of animal science, 2016
[12] Differences in Circulating microRNAs between Grazing and Grain-Fed Wagyu Cattle Are Associated with Altered Expression of Intramuscular microRNA, the …
PLOS ONE, 2016

Copyright © 2025 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.